Module 6 - Microbial Nutrition Flashcards
- Chapt. 11 Metabolic diversity and nutritional types - Chapt 3 Nutrient uptake - Chapt 7 Lab culture of cellular microbes
SA Define Biochemistry
The study of chemical reactions that occur in living systems
SA Name the four main biochemical molecules
Carbohydrates, Lipids, Proteins, Nucleic Acids
SA Where are carbohydrates found
On the cell wall/plasma membrane
SA What is the main use for carbohydrates
Energy
SA Explain + give an example of a monosaccharide
Simple sugars made of 5-6 carbons such as glucose and fructose
SA Explain + give an example of a disaccharide
Two monosaccharides linked through glycosidic bonds such as lactose and sucrose
SA Explain + give an example of a polysaccharide
Polymers made of many linked monosaccharides such as starch and glycogen
T/F Lipids are soluble in water
False, lipids are very soluble in nonpolar solvents but rare in water
SA Name 3 types of lipids
Triacylglycerols (fats), phospholipids, steroids
SA Where are lipids used for
Components in the cell membrane + carbon and energy storage
SA What are proteins
Polymers of amino acids containing at least one amino group and one carboxyl group, linked by peptide bonds. They control nutrient flow and help many processes such as dna replication
SA How many types of amino acids are there + how do we differentiate them?
There are 20 amino acids, all identical with the exception of their “R group” on the central carbon which determines their properties.
SA Explain the 4 types of protein structure
Primary: Line of amino acids joined by peptide bonds
Secondary: Alpha helix or beta sheet, formed by hydeogen bonding
Tertiary: Coiling due to R group interactions
Quaternary: Two or more polypeptide chains associating with each other
T/F Enzymes are specific for substrates
True, specific substrates fit into enzymes like puzzle pieces
SA What are enzymes
Protein catalysts that control the rate of chemical reactions
SA Name the two types of nucleic acid and their differences
DNA: deoxyribose, AGCT bases, double stranded
RNA: ribose, AGCU bases, single stranded
SA What is the most abundant molecule in a living cell?
Water
What does each part of this name mean
Photolithoautotroph
Photo - Energy source
Litho - Electron source
Auto - Carbon source
Explain litho vs organo
Electrons from inorganic vs from organic
Explain photo vs chemo
Energy from light vs from compounds
Explain auto vs hetero
Carbon from CO2 vs organic
What is facilitated diffusion
With the gradient but done with with a carrier protein
What is active transport
Against the gradient with a carrier protein and requires energy
Name 3 functional types of media and 2 main categories
- Supportive - many organisms
- Selective - some microorganisms, inhibits others
- Differential - distinguishes between different groups of microorganisms
- Synthetic - Made in a lab
- Complex - Natural
What is group translocation
Type of active transport used by bacteria and archaea to transport and modify nutrients into a cell
Explain streak plates, spread plates, and pour plates
Streak - Using a spreading tool and sterilizing between streaks
Spread - Using a pipette and spreading tool
Pour - Dilute sample
What is passive diffusion
Diffusion of nutrients into the cell along the concentration gradients