Module 4

Question 1

What temperature are incubators set at?

Answer 1

35°C

Question 2

What are capnophiles?

Answer 2

Bacteria that require a higher CO2 concentration than ambient air.

Question 3

What is the concentration of CO2 in ambient air vs that required by capnophiles?

Answer 3

Ambient- 0.03%

Capnophilic- 3-10%

Question 4

How can you increase the concentration of CO2?

Answer 4

Candle extinction jar (2.5%)

Chemical generation

CO2 incubator

Question 5

What are the different types of bacteria concerning oxygen? Describe their needs.

Answer 5

Obligate aerobes- require atm O2

Obligate anaerobes- can’t grow with O2

Facultative anaerobes- can grow without O2 but grow better with it

Microaerophiles- require less than atm O2

Question 6

What environmental conditions do most human pathogens require?

Answer 6

Meaophilic facultative anaerobes

Question 7

What are the different temperature ranges of bacteria?

Answer 7

Psychrophilic- cold loving, 15-20°C

Mesophilic- middle loving, 25-40°C

Thermophilic- heat loving, 45-50°C