Module 4

Question 1

Temperature Requirements

Answer 1

Minimum, Optimum, Maximum

Lab Incubator temp at 35C to allow 2 degree discrepancy without harming bacteria

Question 2

Psychrophilic

Answer 2

Cold loving

15-20C

Question 3

Mesophilic

Answer 3

Middle loving
25-40C
Most human pathogens are in this category

Question 4

Thermophilic

Answer 4

Heat loving

45-50C

Question 5

Capnophilic

Answer 5

CO2 loving

Require 3-10% CO2

Question 6

Methods for providing increased CO2

Answer 6

Candle Jar, GasPak, CO2 Incubator (gas comes from tank)

Question 7

Obligate Aerobes

Answer 7

Require oxygen for growth

No growth in absence of oxygen

Question 8

Obligate Anaerobes

Answer 8

Only grow in absence of oxygen

Growth inhibited by oxygen (some may be killed)

Question 9

Faculatative Anaerobes

Answer 9

Can grow anaerobically but grow better in presence of oxygen

Most human pathogens are this type

Question 10

Microaerophilic

Answer 10

Require oxygen for growth, but concentration lower than ambient air. Candle jar would provide good conditions but microaerophilic gasp or compressed gas mixture is better.