Module 4

Question 0

What is a Mono layer and why is it important?

Answer 0

A mono layer is layer where rbc are evenly spaces, it’s important because the proper microscopic evaluation depends on the proper cell distribution

Question 1

Peripheral blood smears: good characteristics

Answer 1

3-4cm, thick to thin, thumbprint shaped, free of tails or nicks,

Question 2

Why is staining necessary

Answer 2

Makes the cells visible, differentiate the various cell components

Question 3

3 steps for staining

Answer 3

Fixation, staining, washing

Question 4

What is the purpose of fixation, staining, and washing

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Answer 4

Fixation- kills cells, makes cells permeable to dye, dehydrate cells
Staining- to give cells different colours, so they are easier to identify
Washing? To stop staining

Question 5

Cover slipping protects slides from

Answer 5

Physical damage, deterioration of stain oxidation

Question 6

3’steps of sedimentation

Answer 6

Aggregation, fast settling and packing

Question 7

What is rouleaux?

Answer 7

Aggregation of rbcs in a cylindrical columns