Module 5/6 Flashcards
Order of inoculation
Smear, bap , Mac , broth
Autoclaving standard operating temp, time and pounds?
121, 15 min, 15 pounds
Quality control of autoclave (3 forms)
- autoclave indicator, temp and time recording, biological controls
Sterility checks include
Ph, storage , select medical for functional checks, maintain viable stock cultures , inoculating media, record keeping
Outline gram stain procedure
(VIAS) Violet, Iodine, (alcohol)Decolorlizer, Saffin
Name 2 specimens that require concentration before culturing
Csf, and pleural fluid
Which culture media enchanced growth of most bacteria?
Meat infusions
Sodium chloride does what to the blood agar plate?
Prevents hemolysis
Purple / blue indicates what in gram staining ?
Gram postive
Pink / red indicates what in gram staining ?
Gram negative