Module 2 Flashcards
3 factors that depend on the time required to sediment particles
Rotor speed, radius of rotor , effective path length travelled by particles
Rcf= krn2
K = 1.18x10^-5
R= radius
N=speed of rotation
Instructions when a spill occurs in a centrifuge
turn off and allow it to stop, allow up to 30 min for aerosols to settle, autoclave removable parts, clean inside with cidex, unbroken tubes must be rinsed off with 10,000 ppm , check for glass
Steps for automated tissue processor
Fixation: formalin
Dehydration: graded alcohols
Clearing: xylene
Infiltration: paraffin wax
What is the purpose of the cytocentrifuge ?
To concentrate bacteria and/or cells from liquid specimens
Liquid specimens for cytocentrifuge ?
Csf, synovial, pleural , pericardial, peritoneal
What does h&e stand for?
Hematoxylin and eosin