MODULE 4 Flashcards
The bacterial cell wall is composed of a macromolecular network called
peptidoglycan
Peptidoglycan consists of a repeating _____ attached by ______ to form a lattice that surrounds and protects the entire cell
dissacharide
polypeptide
The disaccharide portion is made up of monosaccharides called
N-acetylglucosamine (NAG)
N-acetylmuramic acid (NAM)
In gram positive cell wall bacteria, the cell wall consists of ___ layers of ______
many layers of peptidoglycan
Gram positive cell wall contain
1.
2.
3.
- teichoic acid
- lipoteichoic acid
- wall teichoic acid
Gram negative cell wall contain only
a thin layer of peptidoglycan
a gel-like fluid between the outer membrane and the plasma membrane.
periplasm
Gram negative cell wall composed of
lipopolysaccharide (LPS)
lipoproteins
phospholipids
is a large complex molecule that contains lipids and carbohydrates
lipopolysaccharide
lipopolysaccharide consists of three components
lipid A
core polysaccharide
O polysaccharide
is the lipid portion of the LPS and is embedded in the top layer of the outer membrane.
Lipid A
lipid A functions as a
endotoxin
is responsible for the symptoms associated with infections by gram-negative bacteria such as fever, dilation of blood vessels, shock, and blood clotting.
lipid A
is attached to lipid A and contains unusual sugars
core polysaccharide
extends outward from the core polysaccharide and is composed of sugar molecules.
O polysaccharide
The O polysaccharide functions as
-antigen
-useful for distinguishing species of gram negative bacteria
stains both gram- positive and gram-negative cells purple because the dye enters the cytoplasm of both types of cells.
crystal violet
forms large crystals with the dye that are too large to escape through the cell wall.
iodine (the mordant)
provides a contrasting color to the primary stain (crystal violet).
safranin
What are the structures internal to the cell wall
plasma membrane
cytoplasm
nucleoid
ribosomes
inclusions
is a thin structure lying inside the cell wall and enclosing the cy- toplasm of the cell
plasma membrane
plasma membranes have
selective permeability
This term indicates that certain molecules and ions pass through the membrane, but that others are prevented from passing through it
selective permeability
What are the permeability factors
size
charge
polarity
are also important to the breakdown of nutrients and the production of energy.
plasma membrane
pigments and enzymes involved in photosynthesis are found in infoldings of the plasma membrane that extend into the cytoplasm.
These membranous structures are called
chromatophores
thylakoids
Materials move across plasma membranes of both prokaryotic and eukaryotic cells by two kinds of processes
passive and active
substances cross the membrane from an area of high concentration to an area of low concentration
passive processes
the cell must use energy (ATP) to move substances from areas of low concentration to areas of high concentration
active processes
Passive processes include
simple diffusion
facilitated diffusion
osmosis
is the net movement of molecules or ions from an area of high concentration to an area of low concentration
simple diffusion
integral membrane proteins function as channels or carriers that facilitate the movement of ions or large molecules across the plasma membrane
facilitated diffusion
integral proteins are called
transporters or permeases
is the net movement of solvent molecules across a selectively permeable membrane from an area with a high concentration of solvent molecules (low concentration of solute molecules) to an area of low concentration of solvent molecules (high concentra9on of solute molecules)
osmosis
What are the three tonicity of the osmosis?
isotonic solution
hypotonic solution
hypertonic solution
No net movement of water occurs.
isotonic solution
Water moves into the cell. If the cell wall is strong,
it contains the swelling. If the cell wall is weak or damaged, the cell bursts (osmotic lysis).
hypotonic
Water moves out of the cell, causing its cytoplasm to shrink (plasmolysis).
hypertonic solution
Among the substances actively transported are
ions
amino acids
simple sugars
for a prokaryotic cell the term refers to the substance of the cell inside the plasma membrane
cytoplasm
cytoplasm is about
80% water
proteins
carbohydrates
lipids
inorganic ions
______ of a bacterial cell usually contains a single long, continuous, and frequently circularly arranged thread of double- stranded DNA called _____
nucleoid
bacterial chromosome
In addition to the bacterial chromosome, bacteria often contain small usually circular, double- stranded DNA molecules called
plasmids
the DNA of prokaryotes is not associated with ____(special chromosomal proteins found in eukaryotes)
histones
prokaryotes divide through
binary fission
Most bacteria range from _____ in diameter
_______ in length
0.2 to 2 um diameter
2 to 8 um in length
basic shapes of bacteria
coccus
bacillus
spiral
usually round but can be oval, elongated, or flaVened on one side.
cocci
Cocci that remain in pairs a[er dividing are called
diplococci
those that divide and remain attached in chainlike patterns are called
streptococci
Those that divide in two planes and remain in groups of four are known as
(cocci)
tetrads
Those that divide in three planes and remain attached in cubelike groups of eight are called
sarcinae
Those that divide in multiple planes and form grapelike clusters or broad sheets are called
staphylococci
divide only across their short axis
bacilli
Most bacilli appear as single rods, called
single bacilli
appear in pairs after division in bacillus
diplobacilli
occurs in chains in bacilli
streptobacilli
others are oval and look
so much like cocci that they are called
coccobacilli
group of spirals that are helical and flexible
spirochetes
spirochetes move by means of
axial filaments
star shaped cells
stella
rectangular flat cells
haloarcula
The shape of a bacterium is determined by
heredity
most bacteria are
monormorphic
they can have many shapes, not just one.
pleomorphic
substance secreted on the surface of prokaryotes.
glycocalyx
meaning ‘sugar coat’, general term used for substances that surround cells
glycocalyx
is a viscous (s9cky), gela9nous polymer that is external to the cell wall and composed of polysaccharide, polypep9de, or both
glycocalyx
glycocalyx is composed of
polysaccharide or polypeptide
it is made inside the cell and secreted to the cell surface.
glycocalyx
If the substance is organized and is firmly attached to the cell wall, the glycocalyx is described as a
capsule
If the substance is unorganized and only loosely attached to the cell wall, the glycocalyx is described as a
slime layer
are important in contributing to bacterial virulence
capsules
the degree to which a pathogen causes disease
virulence
o[en protect pathogenic bacteria from phagocytosis by the cells of the host.
capsules
Bacillus anthracis produces a capsule of
d-glutamic acid
is any group of microorganisms in
which cells s9ck to each other on a surface
biofilm
Biofilms are frequently embedded within a self-produced matrix of
extracellular polymeric substance
is a glycocalyx that helps cells in a biofilm attach to their target environment and to each other.
Extracellular polymeric substance
protects the cells within it, facilitates communica9on among them, and enables the cells to survive by aVaching to various surfaces in their natural environment .
EPS
are long filamentous appendages that propel bacteria.
flagella
bacteria that lack flagella
atrichous
flagella distributed over the entire cell
peritrichous
flagella at one or both poles or ends of the cell
polar
if polar flagella may be?
monotrichous
lophotrichous
amphitrichous
a single flagellum at one pole
monotrichous
a tuft of flagella coming from one pole
lophotrichous
flagella at both poles of the cell
amphitrichous
found in Spirochetes, for motility
axial filaments
bundles of fibrils that arise at the ends of the cell beneath an outer sheath and spiral around the cell
axial filaments or endoflagella
hairlike appendages that are shorter, straighter, and thinner than flagella and are used for attachment and transfer lf DNA rather than for motility
fimbrae
fimbriae is found in ______ bacteria
gram-negative
can occur at the poles of the bacterial cell or can be evenly distributed over the entire cell
fimbriae
help bacteria adhere to epithelial surfaces in the body
fimbriae
are usually longer than fimbriae and number only one or two per cell.
pili
involved in motility and DNA transfer
pili
a pilus extends by the addition of subunits of pilin, makes contact with a surface or another cell, and then retracts (powerstroke) as the pilin subunits are disassembled.
twitching motility
the smooth gliding movement of myxobacteria.
gliding motility
involved in conjugation
pili
Bacteria that look like curved rods
vibrios
have a helical shape, like a corkscrew, and fairly rigid bodies
spirilla
also known as murein
peptidoglycan
Its role is structural — to provide stability.
core polysaccharide