Module 3: Enzymes Flashcards
1
Q
effect of inhibitors of MM parameters
A
comp: Vmax, aKm
uncomp: Vmax/a’, Km/a’
mixed: Vmax/a’, aKm/a’
2
Q
cofactors
A
help in enzyme catalyst
cofactor: inorganic, ionic
coenzyme: organic
3
Q
relaxed state vs tense state
A
R: higher affinity to bind ligands/higher capacity to to catalyze rxn than T
4
Q
cooperative ligand binding
A
change in affinity for a given ligand depending on previous binding events
5
Q
positive cooperativity
A
bindings locks protein in relaxed state
6
Q
negative cooperativity
A
affinity of binding decreases after binding first ligand
7
Q
hemoglobin cooperative binding
A
- positive coop binding
- o2 binding proteins incr amount of o2 in blood
8
Q
hill equation
A
- model cooperative ligand binding
- assumes infinite cooperativity
- n>1: posi coop, n<1: neg coop, n=1: non coop
9
Q
linearize hill equation
A
log(Y/(1-Y))=nlogP(o2)-nlogP(50)
