Module 2: Laboratory Safety Flashcards
Six major categories of hazards
biological hazard chemical hazard electrical hazard fire hazard physical hazard ergonomic hazard
Enacted by the US congress and became basis for many countries in to providing all employees including laboratory personnel a safe work environment
Occupational safety and Health Act
The governing body responsible for ensuring and monitoring the implementation of the
standards set by the OSH Act
Occupational Safety and Health Administrtion
Key elements for safe operation of a clinical laboratory
- formal safety program
- documented policies
- identification kf hazards
- recognition of relevant safety areas of concern
Key important safety items in the laboratory
- proper clothing
- gloves
- eye protection
- eye washer or face washer
- chemical fume hood
Hazards that include all pathogen or disease-causing microorganisms
Biological hazards
Principle of Universal Precaution
treat all blood and blood contaminated sample as potentially infectious, but not pther bodily fluids not visibly contaminated
Principle of Standard precaution
Minimum practice applied to all patient care regardless of status of the patient including, hand hydpgiene, PPPE, cough etiquette, sharps safety, sterile instruments etc
Six Part Chain of Infection Model
Causative agent Reservoir or source Portal of exit Mode of transmission Portal of entry Person at risk
Three Part Chain of infection Model
Source
Transmisssion
Host
Identify which of the Three Part Model is being addressed:
Biohazardous waste disposal
source
Identify which of the Three Part Model is being addressed:
PPE
transmission
Identify which of the Three Part Model is being addressed:
decontamination
source
Identify which of the Three Part Model is being addressed:
aerosol prevention
transmission
Identify which of the Three Part Model is being addressed:
specimen bagging
source
Identify which of the Three Part Model is being addressed:
immunixation
host
Identify which of the Three Part Model is being addressed:
sterile or disposable equipment
transmission
Identify which of the Three Part Model is being addressed:
healthy lifesytle
host
Identify which of the Three Part Model is being addressed:
pest control
transmission
Identify which of the Three Part Model is being addressed:
exposure control plan
host
Identify which of the Three Part Model is being addressed
post exposure prophylaxis:
host
Developed for employees who may be exposed to hazardous chemicals, informing them of health risks associated with those chemicals
Right to Know Law
This is the major source of safety information for employees who may use hazardous materials and should be readily accessible.
MSDS Material Safety Data Sheet
This is the major source of safety information for employees who may use hazardous materials and should be readily accessible.
MSDS Material Safety Data Sheet
What to do when:
Chemical contact with skin and eyes
flush with large amounts of water for at least 15 minutes
Safety showers should be able to dispense:
30-50 gallons per minute at a pressure pf 20-50 psi
Acid and water mixture rule
add acid TO water
Fume hood face velocity
100-120 feet per minute
Store separately:
flammable liquids
flammable solid
Store separately:
organic acids
mineral acids
Store separately:
caustics
oxidizers
Store separately:
perchloric acid
water-reactive substances
Three thiingss stored separately from everything else
- Air reactive substancw
- heat reactive substance requiring refrigeration
- unstable substances
Three thiingss stored separately from everything else
- Air reactive substancw
- heat reactive substance requiring refrigeration
- unstable substances
Required by OSHA to be present at workplaces that handles hazardous chemicals
Chemical Hygiene Plan
A chemical hygieme plan contains the following information:
- standard operating procedures
- utilization of ppe
- engineering controls
- employee training requirements
- medical consultation guidelines
A chemical hygieme plan contains the following information:
- standard operating procedures
- utilization of ppe
- engineering controls
- employee training requirements
- medical consultation guidelines
Responsible for documenting and implementing the chemical hygiene plan
Chemical Hygiene Officer
Refers to susbtances injurious to the skin or eyes by direct contact or to the tissue of the respiratory and GIT if inhaled or ingested.
Corrosive
Cancer-causing chemicals
carcinogenic
Substances that can cause developmental malformations
teratogenic
Substances that, under certain conditions, can spontaneously explode or ignite
reactive
Substances that, under certain conditions, can spontaneously explode or ignite
reactive
Substances that, under certain conditions, can spontaneously explode or ignite
reactive
Substances that, under certain conditions, can spontaneously explode or ignite
reactive
Substances that, under certain conditions, can spontaneously explode or ignite
reactive
temperature at which sufficient vapor is given off to form an ignitable mixture with air.
flash point
Has a flash point below 37°C or 100F (ability to burn easily at normal working temperature)
Flammable
has a flash point at 37°C (100F) or above (ability to burn at temperatures that are usually above normal working temperature)
Combustible liquid
A chemical reaction that involves the rapid oxidation of a combustible material or fuel, with the subsequent liberation of heat and light.
Fire
Components of the fire tetrahedron
a. Fuel
b. Heat or ignition source
c. Oxygen (air)
d. Uninhibited reaction
Components of the fire tetrahedron
a. Fuel
b. Heat or ignition source
c. Oxygen (air)
d. Uninhibited reaction
provides a quick visual representation of the health hazard, flammability, reactivity, and special hazards
NFPA (National Fire Protection Association) diamond or symbol
Color found at the top of the NFPA diamond
red
Red in NFPA symbolizes:
Flammability
Color found at the right of the NFPA diamond
yellow
Yellow in the NFPA diamond represents
reactivity or instability
Color found at the bottom of the NFPA diamond
White
White in the NFPA diamon represents
special hazards
Color found at the left of the NFPA diamond
blue
Blue in the NFPA diamond represents :
Health hazards
0 BLUE
No health hazard
1 BLUE
Cause significant irritatiom
2 BLUE
cause temporary incapacitation or residual injury
3 BLUE
can cause serious or permanent injury
4 BLUE
Can be lethal
0 RED
will not burn
1 RED
> 200F
must be preheated before ignition can occur
2 RED
> 100F
must be heated or high ambient temp to burn
3 RED
<100F
can be ignited under almost all ambient temp
4F
<73F
will vaporize and readily burn at normal temp
1 YELLOW
Stable
1 YELLOW
Normally stable except high temperature
2 YELLOW
violent chemica. change at high temp or pressure
3 YELLOW
May explode at high temp or shock
4 YELLOW
May explode at normal temp or pressure
ALK
alkaline
ACID
Acidic
COR
Corrosive
OX
Oxidizing
☢️
radioactive
₩
reacts violently or explosively wiyh water
₩OX
reacts violently with water and oxidizing
Classification of Fire:
Ordinary combustibles: wood, paper, cloth, plastic
CLASS A
Classification of Fire:
Flammable liquids/gases, gasoline, paint, petroleum
CLASS B
Classification of Fire:
Energized electrical Equipment, motor, switches
CLASS C
Classification of Fire:
Combustible/reactive metals: Mg, Na, K
CLASS D
Classification of Fire:
Liquids used in food preparation such as grease, animal, & vegetable oil
CLASS K
Fire Extinguisher Classes:
soda and acid or water to cool fire
CLASS A extinguisher
Fire Extinguisher Classes:
foam, dry chemical, CO2
CLASS B extinguisher
Fire Extinguisher Classes:
dry chemical, carbin dioxide, halon, non conducting agents to smother fire
CLASS C extinyuisher
Fire Extinguisher Classes:
dry chemical reagent
CLASS ABC extinguishers
Fire Extinguisher Classes:
potassium based alkaline liquid specificlly formulated to gpfight high temperature without splashing
CLASS K
fire extinguisher for CLASS A fires
A, ABC
fire extinguisher for CLASS B fires
B, C, ABC
fire extinguisher for CLASS C fires
B, C, ABC,
BEST: Halon
fire extinguisher for CLASS D fires
Metal X
fire extinguisher for CLASS K fires
K
What to do in case of fire?
R-escue
A-larm
C-ontain
E-xtinguish/ Exit
How to properly use Fire extinguisher
P- ull the pin
A- aim nozzle at the BASE of the fire
S- squeeze the handle or trigger
S- sweep nozzle side to side
examples of physical hazards
heavy electrical equipment
compressed gases
improperly placed machines
Type, wavelength and protective measures for non ionizing radiation from Radiofrequency coil in ICP mass spectrometer
low frequency, 1 cm, engineered shielding and posted pacemaker warning
Type, wavelength and protective measures for non ionizing radiation from Energy Beam microwave used in tissue staining
Microwaves, 0.3cm - 30 cm, engineered shielding
Type, wavelength and protective measures for non ionizing radiation from heat lamp and lasers
infrared, 750 nm- 0.3 cm, containment and warning labels
Type, wavelength and protective measures for non ionizing radiation from genneral illumination amd glare
visible spectrum: 400-750 nm, filters, diffusers, nonreflecrive surfaces
Type, wavelength and protective measures for non ionizing radiation from germicidal lamps in biological safety cabintes
UV, 4-400 nm, eye and skin protection, warning labels
Color-coded garbages:
generl wastes, dry, non infectious
BLACK
Color-coded garbages:
wet, non infectious waste
GREEN
Color-coded garbages:
Wet imfeectious wastes
YELLOW
Color-coded garbages:
sharps
RED (puncture proof containers)
Color-coded garbages:
radioactive wastes
ORANGE
Color-coded garbages:
chemical wastes
YELLOW WITH BLACK BAND
Decontamination solution:
5% lysol
10% sodium hypochlorite
15 MINUTES
disinfection that should be performed daily
sink disinfection with 1:5 or 1:10 sodium hypochlorite
effectivity of sodium hypochlorite stored in plastic bottles
one month if protected from light
proper donning of PPE
- Gown
- Mask
- Goggles
- Gloves
proper doffing of PPE
- gloves
- gown
- goggles
- mask
BLOOD SPILL procedures
- protect immediate area
- wear PPE
- use mechanical device for sharps
- paper towel, gauze pad, tissue to absorb the spill
- 10% bleach fro 20 minutes
- dispose in biohazard container
- dispose gloves
Necessary for medical workers including medical technologists who often extract blood from patients.
Hepa B vaccines
A requirement for the employer to maintain confidential medical records on each employee with occupational exposure
medical records
Medical Records must include
Employee name
Social Security number
HBV vaccination statua
A requirement for the employer to keep a log of injuries from contaminated sharps.
Sharps injury log
sharps injury log must contain
Employee name
date, where, and how incident occured
type and brand of device involved
single most effective way of controlling the spread of infectious diseases
proper handwashing