Exercise 3: Pipettes Flashcards
Pipettes are classified according to:
design, drainage characteristic, type
holds or contains a particular volume but does not dispense that exact volume
TC ( To contain)
pipette that will dispense the volume indicated
TD (to dispense/ deliver)
has continuous etched ring or two small, close continuous rings located near the top of the pipet
blowout pipet
pipette where the content drains by gravity.
self-draining
the last drop of liquid should be expelled into the receiving vessel
blowout pipet
measuring pipette or transfer pipette:
Oswald Folin
Transfer
measuring pipette or transfer pipette:
volumetric
transfer
measuring pipette or transfer pipette:
Mohr
measuring
measuring pipette or transfer pipette:
serologic
measuring
measuring pipette or transfer pipette:
pasteur
transfer
measuring pipette or transfer pipette:
automatic micropipettes and macropipettes
transfer
measuring pipette or transfer pipette:
Bacteriologic
measuring
measuring pipette or transfer pipette:
Ball, Kolmer or Kahn
measuring
measuring pipette or transfer pipette:
micropipette
measuring
can dispense different volumes
Measuring graduated pipet
graduated pipette where graduations end above the tip
Mohr pipette
measuring pipette that has graduated marks down to the tip
serological pipette
the drainage characteristic of Mohr pipette is
self-draining
the drainage characteristic of serological pipette is:
blowout
which has a larger orifice, serologic or mohr?
serologic
It transfers a known volume of liquid without further subdivisions.
transfer pipette
calibrated to deliver accurately a fixed volume of a dilute aqueous solution.
volumetric pipette
have bulb closer to the delivery tip
Oswald Folin
used for accurate measurement of viscous fluids, such as blood or serum
Oswald Folin
drainage characteristic of Oswald Folin:
blowout
drainage characteristic of Volumetric pipetee
self-draining
use of Oswald Folin
biologic fluids with greater viscosity than water
pipettes that do not have calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume and should not be used in any quantitative analytic techniques.
Pasteur pipettes
most routinely used pipet in today’s clinical chemistry laboratory.
automatic pipette
parts of an automatic pipette:
Used to take up and expel liquid
Plunger
parts of an automatic pipette:
pushes the metal bar down which pushes the pipette tip off
eject button
parts of an automatic pipette:
rotated to increase and decrease set volume
volume adjustment
parts of an automatic pipette:
displays set volume and place values shown depending on pipette size
volume window
parts of an automatic pipette:
highest place value which corresponds to size of pipette
top number
parts of an automatic pipette:
lowest place value representing the smallesr you can accurately measure (significant figure)
dashes
parts of an automatic pipette:
tube filled with air or sample liquid
pipette barrel
parts of an automatic pipette:
the first stop is for ______
taking up liquid (set volume
parts of an automatic pipette:
the second stop is for ________
forcing out liquid (extra volume)
parts of an automatic pipette:
sized to fit a specific pipette and changes everytime there’s a risk of contamination
pipette tip
automatic pipettes are clasisfied based on:
delivery volume amount
total volume capacity
mechanism
automatic pipette that dispenses only one volume
fixed volume
pipette models capable of dispensing different volumes
variable volume
automatic pipet capability of less than 1 mL
automaticc micropipet
automatic pipet that dispenses greater than 1 mL
automatic macropipet
Automatic pipette that relies on a piston for creating suction to draw the sample into a disposable tip that must be changed after each use. The piston does not come in contact with the solution
Air-displacement pipette
a method used to compare the reproducibility of the different brands of pipet tips uses
0.1% solution of phenol red in distilled water
Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe, and does not require a different tip for each use (must be rinsed and blotted between samples)
positive displacement
Automatic pipets that obtain the liquid from a common reservoir and dispense it repeatedly
Dilutors/ Dispensers
what pipette to use when:
measuring a volume of 7ml
10 ml
what pipette to use when:
measurring a volume of 3.5 ml
5 ml
what pipette to use when:
measuring a volume of 1.7 ml
2ml
what pipette to use when:
measuring a volume of 0.5 ml
1 ml
immerse the pipette into the solution with the delivery tip about _______ deep
1 inch
