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Clinical Chemistry > Exercise 3: Pipettes > Flashcards

Exercise 3: Pipettes Flashcards

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1
Q

Pipettes are classified according to:

A

design, drainage characteristic, type

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2
Q

holds or contains a particular volume but does not dispense that exact volume

A

TC ( To contain)

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3
Q

pipette that will dispense the volume indicated

A

TD (to dispense/ deliver)

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4
Q

has continuous etched ring or two small, close continuous rings located near the top of the pipet

A

blowout pipet

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5
Q

pipette where the content drains by gravity.

A

self-draining

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6
Q

the last drop of liquid should be expelled into the receiving vessel

A

blowout pipet

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7
Q

measuring pipette or transfer pipette:

Oswald Folin

A

Transfer

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8
Q

measuring pipette or transfer pipette:

volumetric

A

transfer

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9
Q

measuring pipette or transfer pipette:

Mohr

A

measuring

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10
Q

measuring pipette or transfer pipette:

serologic

A

measuring

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11
Q

measuring pipette or transfer pipette:

pasteur

A

transfer

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12
Q

measuring pipette or transfer pipette:

automatic micropipettes and macropipettes

A

transfer

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13
Q

measuring pipette or transfer pipette:

Bacteriologic

A

measuring

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14
Q

measuring pipette or transfer pipette:

Ball, Kolmer or Kahn

A

measuring

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15
Q

measuring pipette or transfer pipette:

micropipette

A

measuring

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16
Q

can dispense different volumes

A

Measuring graduated pipet

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17
Q

graduated pipette where graduations end above the tip

A

Mohr pipette

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18
Q

measuring pipette that has graduated marks down to the tip

A

serological pipette

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19
Q

the drainage characteristic of Mohr pipette is

A

self-draining

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20
Q

the drainage characteristic of serological pipette is:

A

blowout

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21
Q

which has a larger orifice, serologic or mohr?

A

serologic

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22
Q

It transfers a known volume of liquid without further subdivisions.

A

transfer pipette

23
Q

calibrated to deliver accurately a fixed volume of a dilute aqueous solution.

A

volumetric pipette

24
Q

have bulb closer to the delivery tip

A

Oswald Folin

25
Q

used for accurate measurement of viscous fluids, such as blood or serum

A

Oswald Folin

26
Q

drainage characteristic of Oswald Folin:

A

blowout

27
Q

drainage characteristic of Volumetric pipetee

A

self-draining

28
Q

use of Oswald Folin

A

biologic fluids with greater viscosity than water

29
Q

pipettes that do not have calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume and should not be used in any quantitative analytic techniques.

A

Pasteur pipettes

30
Q

most routinely used pipet in today’s clinical chemistry laboratory.

A

automatic pipette

31
Q

parts of an automatic pipette:

Used to take up and expel liquid

A

Plunger

32
Q

parts of an automatic pipette:

pushes the metal bar down which pushes the pipette tip off

A

eject button

33
Q

parts of an automatic pipette:

rotated to increase and decrease set volume

A

volume adjustment

34
Q

parts of an automatic pipette:

displays set volume and place values shown depending on pipette size

A

volume window

35
Q

parts of an automatic pipette:

highest place value which corresponds to size of pipette

A

top number

36
Q

parts of an automatic pipette:

lowest place value representing the smallesr you can accurately measure (significant figure)

A

dashes

37
Q

parts of an automatic pipette:

tube filled with air or sample liquid

A

pipette barrel

38
Q

parts of an automatic pipette:

the first stop is for ______

A

taking up liquid (set volume

39
Q

parts of an automatic pipette:

the second stop is for ________

A

forcing out liquid (extra volume)

40
Q

parts of an automatic pipette:

sized to fit a specific pipette and changes everytime there’s a risk of contamination

A

pipette tip

41
Q

automatic pipettes are clasisfied based on:

A

delivery volume amount
total volume capacity
mechanism

42
Q

automatic pipette that dispenses only one volume

A

fixed volume

43
Q

pipette models capable of dispensing different volumes

A

variable volume

44
Q

automatic pipet capability of less than 1 mL

A

automaticc micropipet

45
Q

automatic pipet that dispenses greater than 1 mL

A

automatic macropipet

46
Q

Automatic pipette that relies on a piston for creating suction to draw the sample into a disposable tip that must be changed after each use. The piston does not come in contact with the solution

A

Air-displacement pipette

47
Q

a method used to compare the reproducibility of the different brands of pipet tips uses

A

0.1% solution of phenol red in distilled water

48
Q

Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe, and does not require a different tip for each use (must be rinsed and blotted between samples)

A

positive displacement

49
Q

Automatic pipets that obtain the liquid from a common reservoir and dispense it repeatedly

A

Dilutors/ Dispensers

50
Q

what pipette to use when:

measuring a volume of 7ml

A

10 ml

51
Q

what pipette to use when:

measurring a volume of 3.5 ml

A

5 ml

52
Q

what pipette to use when:

measuring a volume of 1.7 ml

A

2ml

53
Q

what pipette to use when:

measuring a volume of 0.5 ml

A

1 ml

54
Q

immerse the pipette into the solution with the delivery tip about _______ deep

A

1 inch

Clinical Chemistry (14 decks)

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