Module 2: Cell Structure Flashcards

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1
Q

What is resolution determined by?

A

Wavelength of light/beam of electrons.

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2
Q

Define magnification.

A

How many time larger the image is compared to the object.

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3
Q

Describe a dry mount.

A

Thin slices or whole specimens on a slide with a cover slip onto.

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4
Q

Give an example of a specimen that a dry mount would be used for.

A

*plant tissue
*hair

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5
Q

Describe a wet mount.

A

Specimens added to water/stain before lowering cover-slip on top with a mounted needle.

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6
Q

Why is the cover-slip lowered onto a wet mount with a mounted needle?

A

To prevent air bubbles forming.

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7
Q

Give an example of a specimen that a wet mount is used for.

A

Aquatic organisms.

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8
Q

Describe a squash slide.

A

Wet mounds with a cover-slip pushed on top.

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9
Q

What is the need for a squash slide?

A

Squash specimen to create thin layer of cells so light can pass through.

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10
Q

Give an example of a specimen that a squash slide is used for.

A

Root tip.

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11
Q

Describe a smear slide.

A

The edge of another slide smears the sample across the slide with cover-slip on top.

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12
Q

What is the need for a smear slide?

A

Creates a smooth, thin, even coating of the specimen.

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13
Q

Give an example of a specimen a smear slide would be used for.

A

Blood smear.

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14
Q

What is an eyepiece graticule?

A

A scale in a microscope used to measure specimens.

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15
Q

When do you have to calibrate an eye piece graticule?

A

Every time the lens is changed.

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16
Q

Explain how to calibrate an eyepiece graticule.

A
  1. line up stage micrometer and eyepiece graticule whilst looking through the eyepiece
    2, count how many divisions on the eyepiece graticule fit into 1 division on the micrometer scale
  2. each division on micrometer scale is 10 μm, so calculate what 1 division on eyepiece graticule is at that magnification
17
Q

What is the equation for image size?

A

Image size = actual size x magnification

18
Q

What is the purpose of staining samples?

A

Makes it easier to see, makes parts stand out, make parts contrast.

19
Q

What is differential staining?

A

Staining involving many chemicals to stain different parts different colours.

20
Q

What do crystal violet and methylene blue stain?

A

Negatively charged parts of the cell.

21
Q

What do nigrosin and congo red stain?

A

Background