Module 1.1: Basic Microscopy Techniques and Virtual Microscopy

Question 1

What are the three types of lenses in a light microscope?

Answer 1

Ocular, Objective, Condenser

Question 2

Does the diameter of an image increase or decrease when the objective len’s magnification increases?

Answer 2

If magnification INCREASES, diameter of field of view DECREASES

Question 3

Identify the 4 types of microscopes.

Answer 3

Simple, Compound Light, Stereo, Confocal

Question 4

Describe the specimen appearance for the following: Bright-field, Dark-field, Phase-contrast, Fluorescence

Answer 4

Bright-field: Light background, stained specimen
Dark-field: Dark background, light specimen
Phase-contrast: Grey background, distinct organelles
Fluorescence: Fluorescent-dyed specimen, Dark background, UV RAYS

Question 5

Identify the two types of electron microscopes and their major distinction.

Answer 5

Scanning Electron Microscope: Depicts surface of the cell
Transmission Electron Microscope: Depicts transverse image of the cell

Question 6

What are the two major component groupings of a compound microscope?

Answer 6

Mechanical & Optical parts

Question 7

What are the two groupings of optical components of a compound microscope?

Answer 7

Illuminating & Magnifying parts

Question 8

What are the strength of magnifications for an LPO, HPO and Scanner objective lens?

Answer 8

LPO: 10x
HPO: 45x
Scanner: 4x

Question 9

Define Parfocal lenses

Answer 9

Lenses that stay in focus when magnification is changed.

Question 10

Identify the two types of specimen and define their preparations.

Answer 10

Prepared: dehydrated, cut, mounted stained.
Wet-mound: specimen is living; liquid-y when slide is mounted