MODULE 10 Flashcards
The act of modifying genes in a living organism to produce genetically modified organisms (GMOs)
GENETIC ENGINEERING
GMOS, ALSO KNOWN AS…
TRANSGENIC ORGANISMS
An application of biotechnology that uses biological systems, processes, or organisms to create products to improve quality of human life
GENETIC ENGINEERING
____ is physically removed and placed in an organism to be modified
GENE OF INTEREST
4 STEPS OF GENETIC ENGINEERING
- DNA ISOLATION
- LIGATION
- TRANSFORMATION
- INTEGRATION, EXPRESSION, AND INHERITANCE
STEP OF GENETIC ENGINEERING: isolating plasmid and gene of interest
DNA ISOLATION
STEP OF GENETIC ENGINEERING: gene insertion to the plasmid. Resistant gene is inserted into isolated plasmid, cleaved using the same restriction enzyme before being combined using DNA ligases
LIGATION
used to cut/melt the DNA. WHEN they recognize a specific nucleotide sequence, they cleave the strands.
RESTRICTION ENZYMES
Restriction Enzymes are also called…
RESTRICTION ENDONUCLEASES
USED to glue/combine the DNA
DNA LIGASES
plasmid is placed back to the bacterial cell. recombinant plasmid would be placed back to the bacterium.
TRANSFORMATION
____ of gene into recipient genome; ____ of gene in recipient genome; and ____ of gene through further generations
- INTEGRATION
- EXPRESSION
- INHERITANCE
Genes of bacteria, plants, and animals are modified to…
IMPROVE THE QUALITY OF HUMAN LIFE
GMOs have many uses in:
- AGRICULTURE
- MEDICINE
- ENVIRONMENT
2 GENETICALLY MODIFIED BACTERIA THAT HAS USES
- ESCHERICHIA COLI
- CYANOBACTERIA