Module 1 - The Microbial World Flashcards
What is microbiology?
The study of microorganisms
What are some examples of microorganisms?
Bacteria, protozoa, and fungi
True or false: microbes can be seen with the naked eye
False: most cannot be seen with the naked eye
Why do we need to study microbiology?
Microbes have a complex and dynamic relationship with humans
How can bacteria be harmful to humans?
By causing infectious diseases
How can bacteria be helpful to humans?
By aiding in digestion and immune system development
True or false: microorganisms only affect animals
False: they can also impact plants
Where is microbiology used?
In biotechnology and the food industry
How is microbiology used in biotechnology?
By using microbes to create drugs
How is microbiology used in the food industry?
By creating food such as milk, butter, bread, and cheese
What is the definition of life?
A self-organizing, self replicating, non-equilibrium system
What is the purpose of life?
To preserve and reinforce its existence in the environment
What are some characteristics of life?
Metabolism, growth, reproduction, genetic variation/evolution, response/adaptation to the external environment, homeostasis
What macromolecules are needed for life?
Proteins, lipids, carbohydrates, and nucleic acids
What are the subunits of polypeptides?
Amino acids
What are the subunits of nucleic acids?
Deoxyribonucleotides and ribonucleotides
What are the subunits of lipids?
Diverse structures
What are the subunits of carbohydrates?
Sugars
What are the functions of proteins?
Catalyze biochemical reactions (enzymes) and act as structural components
What are the functions of nucleic acids?
Store hereditary information (DNA), and produce polypeptides (RNA)
What are the functions of lipids?
Make up cell membrane to separate interior and exterior
What are the functions of polysaccharides?
Energy storage (glycogen, starch) and structure (chitin, cellulose)
What are the most abundant molecules in cells?
Proteins and polypeptides
Which are more abundant in a cell: RNA or DNA?
RNA
What is the difference between a protein and a polypeptide?
The function
How is the shape of a polypeptide different than a protein?
Long polypeptide chains fold into proteins, which have a well defined structure
What is the function of the cell membrane?
Separate external and internal cell compartments
Before the 1970s, how were organisms classified?
As prokaryotes or eukaryotes
What are prokaryotes?
Organisms with no membrane-bound organelles
What are eukaryotes?
Organisms with membrane-bound organelles
Today, how are organisms classified?
Into 3 domains
What are the 3 domains of life?
Bacteria, archaea, and eukarya
What is the basis for the 3 domains of life?
Differences in the 16S rRNA gene
In a phylogenetic tree of life, what does the linear distance represent?
Proportional to the sequence similarity of the 16S rRNA gene in those two organisms (evolutionary distance)
How has the tree of life been updated recently (2016)?
By analyzing 16 ribosomal proteins other than the 16S rRNA gene
What is the significance of the 16S rRNA gene?
Determines the tree of life evolutionary distance through sequence analysis
What is the purpose of using 16 ribosomal proteins (instead of the 16S rRNA gene) for the tree of life?
Updated resolution
True or false: bacteria have a nuclear membrane
False
True or false: archaea have a nuclear membrane
False
True or false: eukarya have a nuclear membrane
True
True or false: bacteria commonly have membrane-bound organelles
False: it is rare, only found in a few species
True or false: archaea commonly have membrane-bound organelles
False: it is rare, only found in a few species
True or false: eukarya commonly have membrane-bound organelles
True: multiple distinct types are found in all species
Which domain(s) have a nuclear membrane?
Eukarya
Which domain(s) have membrane-bound organelles?
Eukarya
Describe the plasma membrane of bacteria (in terms of the other domains)
Similar to eukarya
Describe the plasma membrane of eukarya (in terms of the other domains)
Similar to bacteria
Describe the plasma membrane of archaea (in terms of the other domains)
Different from eukarya and bacteria
Which domains have similar plasma membranes?
Bacteria and eukarya
Describe the cell wall of bacteria (generally)
Found in nearly all species, made up of peptidoglycans
Describe the cell wall of archaea (generally)
Found in nearly all species, made up of various materials
Describe the cell wall of eukarya (generally)
Found in some species, made up of various materials
How many RNA polymerases do bacteria have?
One
How many RNA polymerases do eukarya have?
Three (RNA pol I, II, and III)
How many RNA polymerases do archaea have?
One (Eukaryal-like RNA pol II)
True or false: bacteria have histones
False: they have histone-like proteins
True or false: archaea have histones
True
True or false: eukarya have histones
True
Which domain(s) have histones?
Archaea and eukarya
What was the early environment on Earth like?
Little oxygen, and a soup of chemicals
What happened to the soup of chemicals in early Earth?
They became macromolecules and single cells
When did oxygen producing bacteria come into existance?
About 3 billion years ago
When was the oxygen atmosphere established on Earth?
About 2 billion years ago
How were eukaryotes formed on early Earth?
Through endosymbiosis
What is the endosymbiotic theory?
Primitive prokaryotic microbes ingested other microbes, starting a symbiotic relationship, forming the first basic eukaryotes
What does the endosymbiotic theory describe?
How eukaryotic organisms first appeared on early Earth from prokaryotic organisms
How did the two organisms interact under the endosymbiotic theory?
One lived inside the other, with them both acting as a single organism
What does symbiosis mean?
The interaction between two different organisms living in close physical association, usually to the advantage of both
Which organelles arose from an endosymbiotic process?
Chloroplasts and mitochondria
How did mitochondria benefit other cells?
By using oxygen to produce chemical energy (respiration)
How did chloroplasts benefit other cells?
By fixing carbon to create sugar molecules (photosynthesis)
How long have bacteria dominated the Earth?
About 3.5 billion years
What was the purpose of the Miller-Urey experiment?
To show that organic molecules could arise from early Earth conditions
When was the Miller-Urey experiment performed?
In the 1950s
What is the purpose of water in the Miller-Urey experiment?
It was critical to convert inorganic molecules into organic molecules
What was the purpose of the spark in the Miller-Urey experiment?
Simulate lightning that could be a source of energy for the biochemical reactions to occur
What was the conclusion of the Miller-Urey experiment?
Organic molecules can be formed with light and water
What are some fundamental questions (4) regarding the origin of life?
- How are macromolecules produced?
- What was the source of genetic information?
- What catalyzed biochemical reactions?
- What separated interior from exterior?
Where is iron found on Earth?
In the inner core
What was the significance of iron in the early Earth?
It could act as a surface for molecules to stick to and form macromolecules
What is needed for a cell to replicate?
Biochemical reactions, and a genetic component
What are ribozymes?
RNA that can catalyze biochemical reactions
How come RNA could support independent life forms?
They could act as a catalyst for biochemical reactions, and a genetic component (dual purpose for replication)
What was an early form of the plasma membrane?
Micelles
What are micelles?
A single layer of phospholipids that can separate interior from exterior
How come plasma membranes are composed of a bilayer, and not a monolayer?
The size of a micelle is more limited than a bilayer
What were the components of the earliest microbes?
Micelles and RNA
Why is DNA used to store hereditary information now instead of RNA?
DNA has a backup copy (double strand), and it is more stable than RNA
What are the advantages of microbes (4 reasons)?
- Fast and easy to grow
- Produce enzymes and other molecules for industrial/medical uses
- Simpler to study (less genes)
- Easier genetic manipulation (compared to eukaryotic cell)
How long does E. coli take to divide?
20 min
How long does yeast take to divide?
90 min
Before microbes were discovered, what was believed to be the source of diseases?
Angry gods or bad air
How did microbiology begin?
Through the development of the microscope
What did Anton van Leeuwenhoek do?
First observed microbes with 300x magnification lens
Who is considered the father of microbiology?
Anton van Leeuwenhoek