Module 1: Hormones

Question 1

Competitive immunoassay

Answer 1

Labeled reagent antigen (tracer) and unlabeled patient analyte compete for binding sites
Typically only one capture antibody

Question 2

Non-competitive immunoassay

Answer 2

“Sandwich”
Analyte binds to capture antibody
A second labeled antibody (conjugate) binds to analyte

Question 3

Heterogenous immunoassay

Answer 3

Signal from label is the same whether bound or not

Must separate bound from unbound (usually washing)

Question 4

Homogenous immunoassay

Answer 4

Signal from label changes when bound

No need to separate bound from unbound