Lab exam Flashcards
Specimens that can be run on IFE
Serum, urine, CSF
Purpose of a reagent blank
To account for the colour of the reagent
Composition of a reagent blank
Reagent and diluent (water) subsituted for sample
Purpose of a sample blank
To account for the colour of the sample
Composition of a sample blank
Sample and diluent (water) substituted for reagent
Purpose of standards (calibrators)
To produce a calibration curve to accurately determine unknown results
Accuracy
Nearness to true value
Precision
Reproducibility over time
Purpose of controls
To detect error
Basic electrophoresis process
- Sample is applied to gel at the point of application
- Gel is placed in chamber filled with buffer
- Electric current applied by power supply
- Proteins migrate in the electrical field. After a specified period of time, power is turned off and gel is removed
- The gel is dried or fixed, then stained
In SPE, what would be the effect of using a buffer of higher ionic strength?
Increased resolution of bands
Slower overall migration
Composition of controls
Same matrix as patient samples
Composition of standards (calibrators)
Pure substance dissolved in water with no other compounds present
Principle used by colorimetric methods on the Vitros
All reagents are contained on a dry slide which acts as the cuvet; Basically reverse photometry with a mirror; Reflectance is inversely proportional to concentration (nonlinear)
Layers of a dry slide (Vitros)
Spreading/ Reflecting Layer - distributes sample evenly on slide; removes large molecules that may interfere (eg. proteins and lipids); makes a protein-free filtrate; reflects light transmitted through lower layers
Reagent/Indicator Layer - contains the reagents necessary for chemical reaction to produced colored product
Support Layer - supports upper layers; transparent to allow light through for measurement
