Modern Techniques Flashcards
1
Q
A collection of cloning vectors with DNA inserts that cover some part of a genome
A
Genomic Library
2
Q
A cDNA library of expressed genes
A
Expression library
- Requires primer to match poly-A tail
- Digest all remaining RNA
- Use DNA Polymerase I
- Use S1 nuclease to cleave the turn at the end of the 3’ end
3
Q
SNP
A
Single Nucleotide Polymorphism
- A single nucleotide change in a DNA sequence that can be compared to other people’s DNA
- Allows us to find nucleotide responsible for mutant alleles, such as with sickle cell anemia
4
Q
FISH
A
Fluorescent in situ hybridization
-Biotinylated linkers are used in a DNA probe, which allows us to visualize copies of a gene in a cell or on a karyotype
5
Q
Paint Probes
A
Use biotinylated linkers with multiple DNA probes for an entire chromosome to look for translocations
6
Q
EST
A
Expressed Sequence Tag
