Bacterial Recombination

Question 1

Conjugation

Answer 1

Parasexual process that transfers DNA through pili; DNA is replicated as a single strand and made doubled stranded in recipient cell

Question 2

Transformation

Answer 2

Uptake of a DNA molecule; only possible by competent bacteria; one strand is broken down as it enters and the other strand can:

1. Get broken down
2. Circularize into a plasmid, if a full plasmid
3. Pair with homologous region on chromosome and undergo single stranded recombination

Question 3

Transduction

Answer 3

Gene movement through viral vector

• Generalized: DNA is encapsulated into viral proteins
• Specialized: DNA is incorporated into viral genome

Question 4

An exact copy of a petri dish with colonies

Answer 4

Replica Plating

Question 5

F-Plasmid

Answer 5

Plasmid with genes for conjugation
F+ has at least one F-plasmid
F- lack F-plasmid

Question 6

Hfr Conjugation

Answer 6

Starts at oriT (opposite the direction of arrow head) and transfers chromosomal DNA and is recombined as double-stranded DNA