MLT BB antibody detection and ID Flashcards
When performing antibody ID which facts about the patient may be helpful:
Transfusion history
Pregnancy history
Race
Clinical diagnosis
martial status N/A
Antibodies that generally react at the room temp phase of testing:
Anti-M, N, Lea, Leb, P1
Differences in strength of reactions among panel cells within the phase of testing may indicate:
Multiple antibodies
Antibody showing dosage
Which blood group antigen is most susceptible to denaturization from the action of proteolytic enzymes, such as papain and ficin?
Fya, Fyb
Duffy
How is the elution of antibodies from rbc’s generally accomplished?
disruption of the chemical bonds between the antigen-antibody complexes
List three techniques that are helpful in the investigation of a patient with multiple antibodies:
- Perform select cell panel; to exclude certain specificities
- Enzyme enhancement; allows for separation of antibody if one antigen is destroyed by enzymes and another has enhanced expression or is unaffected by enzyme treatment
- Neutralization or adsorption techniques; may prove useful in separating multiple antibodies
Equation to determine how many units of blood must be screened for compatibility:
(units needed)/([anti frq]*[anti Frq]…)
round up
Multiple Myeloma
rouleaux
M ntigen
antigen demonstrating dosage
Autoantibody with possible Rh specificity
warm autoimmune hemolytic anemia
possible autoanti-I
cold autoimmune hemolytic anemia
In vivo rbc Ag-Ab reaction
pos DAT
In vitro Ag-Ab reaction
pos Antibody detection(screen) test
Transfused rbc
mixed field agglutination
An antibody demonstrating dosage would meant that:
homozygous cells were stronger
