MLT 259 BB Study Guide Flashcards
List antibodies that may cause HDN
ABO, RH, Kell, Duffy
List antibodies that may cause delayed HTR
Kidd
If R2R2 patient has a 3+ reaction and is compatible with R1r donor, what antibody is present?
anti-E
How are cold agglutinates and rouleaux excluded from testing?
Cold - absorption
Roul - saline replacement
List antibodies, detected at different phases
AHG - Kell, Duffy, Kidd, MNS, Lutheran
IS - Lewis, MN, P1
37 - Rh, MN
A. interval between donations
a. 8 weeks
B. Hgb
b. greater than or equal to 12.5 g/dL
C. Hct
c. greater than or equal to 38%
D. total volume of blood drawn
d. 450 mL
525 mL max
E. permanent deferrals
e. positive for HBsAg
F. weight
f. greater than or equal to 110
G. deferral for malaria
g. one year
H. Max body temp
37.5C or 99.5F
Mom is R1r
child 1 is rr
child 2 is R2r
What is dad?
R2r
What blood type is used for emergency release and alternate blood selection?
O neg
Blood storage refrigerator temp
1-6 C
Length of time recipient samples are stored
7 days
Shelf life after hermetic seal is broke
24 hours
Temp range during whole blood shipment
1-10 C
Criteria of discarding blood units
hemolyzed
What is the order of re-activity of ABO blood groups with anti-H?
O, A2, B, A2B, A1, A1B
Hemphiliac patient would need what type of blood component?
Cryo
*contains concentrated fibrinogen and Factor VIII
What is unique about the Lewis System?
- Only blood group system not manufactured by RBCs.
- Can show in saliva and tears
- Pregnant women may present Le(a-b-) and produce Lewis antibodies even though true phenotype is Le(a-b+)
What antibodies are found in Bombay individuals?
anti-A, B, AB, H
Panel study
choose 2 negative and 1 positive for both antibodies
What blood group antigens are enhanced by enzymes?
Lewis Rh Kidd Ii P
What blood group antigens are destroyed by enzymes?
Fya, Fya, MNS
What would you expect if a Rh neg pt who has never been transufed or pregnant, was transfused with Rh pos blood?
No reaction; no antibodies are present b/c there has been no prior exposure
Who should Rhogam be given?
When?
Rh neg mom, Rh pos baby
28 weeks gestation, 72 hrs within delivery
Used to remove Ab bound to sensitized rbcs; determine alloAb, autoAb, or HDN
elution
using serum antibodies to detect unknown antigens
detect presence or absence of Ag
Forward typing
In vivo
used to detect Ab attached to pt rbc
when +, use elution to remove Ab to ID specific eluate used to diagnose HDN, HTP, AIMA
DAT
Anti-IgG and anti C3d
polyspecific AHG
Anti-A1 lectin is used to detect what?
A1 subgroups
- ABO discrepancy -
Found on cord blood
Wharton’s jelly
Why are reverse typings done on newborns?
Antibodies are not yet developed
chills, fever, increase temp by 2C
febrile reaction
What could a weak subgroup of A cause?
ABO discrepancy
What would you use to distinguish between an A1/A2 discrepancy?
Dolichus Biflorus
If mom is O and the baby is A and there is HDN, what would you transfuse with?
O
Would A2B with anti-A1 cause a discrepancy?
Yes
What would the next step be to ID the cause of a strong positive DAT in cord blood and mother sample?
perform an elution
Why are the cells washed in the AHG test?
to prevent neutralization by globulins
What is the reason for a mf reaction on a DAT for a pt who has been previously transfused?
2 cell populations in the patients blood
What type of sample do you need for compatibility testing?
Why?
FRESH serum
to preserve complement
What type of elution is required for HDN?
acid elution; if not ABO incompatibility
ARIPLKidd
antibodies enhanced by enzymes
MNS, Duffy, Xga
antibodies, destroyed, inactivated by enzymes
Kell is not affected
