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MLT Clinical Practicums > MLT 259 BB Study Guide > Flashcards

MLT 259 BB Study Guide Flashcards

1
Q

List antibodies that may cause HDN

A

ABO, RH, Kell, Duffy

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2
Q

List antibodies that may cause delayed HTR

A

Kidd

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3
Q

If R2R2 patient has a 3+ reaction and is compatible with R1r donor, what antibody is present?

A

anti-E

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4
Q

How are cold agglutinates and rouleaux excluded from testing?

A

Cold - absorption

Roul - saline replacement

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5
Q

List antibodies, detected at different phases

A

AHG - Kell, Duffy, Kidd, MNS, Lutheran
IS - Lewis, MN, P1
37 - Rh, MN

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6
Q

A. interval between donations

A

a. 8 weeks

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7
Q

B. Hgb

A

b. greater than or equal to 12.5 g/dL

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8
Q

C. Hct

A

c. greater than or equal to 38%

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9
Q

D. total volume of blood drawn

A

d. 450 mL

525 mL max

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10
Q

E. permanent deferrals

A

e. positive for HBsAg

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11
Q

F. weight

A

f. greater than or equal to 110

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12
Q

G. deferral for malaria

A

g. one year

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13
Q

H. Max body temp

A

37.5C or 99.5F

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14
Q

Mom is R1r
child 1 is rr
child 2 is R2r

What is dad?

A

R2r

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15
Q

What blood type is used for emergency release and alternate blood selection?

A

O neg

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16
Q

Blood storage refrigerator temp

A

1-6 C

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17
Q

Length of time recipient samples are stored

A

7 days

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18
Q

Shelf life after hermetic seal is broke

A

24 hours

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19
Q

Temp range during whole blood shipment

A

1-10 C

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20
Q

Criteria of discarding blood units

A

hemolyzed

21
Q

What is the order of re-activity of ABO blood groups with anti-H?

A

O, A2, B, A2B, A1, A1B

22
Q

Hemphiliac patient would need what type of blood component?

A

Cryo

*contains concentrated fibrinogen and Factor VIII

23
Q

What is unique about the Lewis System?

A
  • Only blood group system not manufactured by RBCs.
  • Can show in saliva and tears
  • Pregnant women may present Le(a-b-) and produce Lewis antibodies even though true phenotype is Le(a-b+)
24
Q

What antibodies are found in Bombay individuals?

A

anti-A, B, AB, H

25
Q

Panel study

A

choose 2 negative and 1 positive for both antibodies

26
Q

What blood group antigens are enhanced by enzymes?

A 
Lewis
Rh
Kidd
Ii
P
27
Q

What blood group antigens are destroyed by enzymes?

A

Fya, Fya, MNS

28
Q

What would you expect if a Rh neg pt who has never been transufed or pregnant, was transfused with Rh pos blood?

A

No reaction; no antibodies are present b/c there has been no prior exposure

29
Q

Who should Rhogam be given?

When?

A

Rh neg mom, Rh pos baby

28 weeks gestation, 72 hrs within delivery

30
Q

Used to remove Ab bound to sensitized rbcs; determine alloAb, autoAb, or HDN

A

elution

31
Q

using serum antibodies to detect unknown antigens

detect presence or absence of Ag

A

Forward typing

32
Q

In vivo
used to detect Ab attached to pt rbc

when +, use elution to remove Ab to ID specific eluate used to diagnose HDN, HTP, AIMA

A

DAT

33
Q

Anti-IgG and anti C3d

A

polyspecific AHG

34
Q

Anti-A1 lectin is used to detect what?

A

A1 subgroups

- ABO discrepancy -

35
Q

Found on cord blood

A

Wharton’s jelly

36
Q

Why are reverse typings done on newborns?

A

Antibodies are not yet developed

37
Q

chills, fever, increase temp by 2C

A

febrile reaction

38
Q

What could a weak subgroup of A cause?

A

ABO discrepancy

39
Q

What would you use to distinguish between an A1/A2 discrepancy?

A

Dolichus Biflorus

40
Q

If mom is O and the baby is A and there is HDN, what would you transfuse with?

A

O

41
Q

Would A2B with anti-A1 cause a discrepancy?

A

Yes

42
Q

What would the next step be to ID the cause of a strong positive DAT in cord blood and mother sample?

A

perform an elution

43
Q

Why are the cells washed in the AHG test?

A

to prevent neutralization by globulins

44
Q

What is the reason for a mf reaction on a DAT for a pt who has been previously transfused?

A

2 cell populations in the patients blood

45
Q

What type of sample do you need for compatibility testing?

Why?

A

FRESH serum

to preserve complement

46
Q

What type of elution is required for HDN?

A

acid elution; if not ABO incompatibility

47
Q

ARIPLKidd

A

antibodies enhanced by enzymes

48
Q

MNS, Duffy, Xga

A

antibodies, destroyed, inactivated by enzymes

Kell is not affected

MLT Clinical Practicums (12 decks)

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