MLSCI340 Mycology Flashcards

1
Q

Discuss collection and submission of specimens for fungal examination

A
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2
Q

Discuss media used for isolation and/or ID of fungi

A
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3
Q

Discuss clinical significance of organisms listed in lab

A
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4
Q
A
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5
Q

List the dimorphic forms of fungi

A

Smooth, creamy OR fluffy, powdery

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6
Q

Discuss respiratory specimen collection/ handling in mycology

A
  • collected immediately in the morning
  • cough deeply from lungs (NOT SALIVA) = THICK, not watery
  • sputum must arrive in lab within 2 hours of collection
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7
Q

What temp are respiratory mycology specimens stored if not immediately sent to lab ?

A

4°C in fridge

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8
Q

Describe blood specimen collection/ handling in mycology ?

A
  • 70% alcohol antiseptic
  • 2 bottles (aerobic and *) at 2 sites
  • If dimorphic, 10 mL in SPS or isolator tube = held RT for 4 weeks

SPS - Sodium polyanethole sulfonate

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9
Q

Discuss tissue collection / handling in mycology

A
  • sterile technique + gauze moistened w/ sterile saline
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10
Q

Discuss CSF/ sterile body fluid collection/ handling in mycology

A
  • 2 sterile 10 mL tubes (2nd tube is submitted to micro)
  • Fungal culture includes cryptococcal Ag testing + calcofluor white stain
  • India ink stain used to ID large capsules seen with Cryptococcus
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11
Q

Discuss MSU collection/ handling in mycology

A
  • first morning collection
  • ensure proper disinfection to remove normal flora
  • urine into toilet before and after collection
  • grey top vacutainer is okay at RT; 4°C in fridge if >20 min
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12
Q

Discuss hair specimen collection/ handling in mycology

A
  • fungal collection kit (envelope, black paper)
  • forcep >10 hair (uncut)
  • clean scalp not required

Hx:
- anti-fungal therapy
- travel
- predisposing factors (animal contact, immunodeficiency)

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13
Q

Discuss skin specimen collection/ handling in mycology

A
  • fungal collection kit (envelope, black pepper)
  • disinfect with 70% alcohol
  • scrape with sterile scalpel

Hx:
- anti-fungal therapy
- travel
- predisposing factors (animal contact, immunodeficiency)

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14
Q

Discuss nail specimen collection/ handling in mycology

A
  • fungal collection kit (envelope, black paper)
  • clean w/ 70% alcohol
  • use scalpel to remove debris from under nail
    Hx:
  • anti-fungal therapy
  • travel
  • predisposing factors (animal contact, immunodeficiency)
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15
Q

SDA use and selective ingredients

A

Sabouraud Dextrose Agar

Use: ENRICHED; primary recovery of saprobes (including dermatophytes)

Ingredients: sabouraud dextrose + brain-heart infusion agar

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16
Q

What is SDA with anti-microbials used for ?

A

Selective between different saprobes

17
Q

BHI Agar use and ingredients

A

Brain-Heart Infusion Agar

Use: primary recovery of saprobes (bacteria, yeast, fungi)
Ingredients: Brain-heart infusion, enzymatic digest of animal tissue, casein, dextrose, NaCl

18
Q

Why may BHI be used with anti-microbials?

A

It is a rich medium for saprobes

19
Q

WIP What is the difference between saprobes and dermatophytes ?

A