MLSCI340 Mycology Flashcards
Discuss collection and submission of specimens for fungal examination
Discuss media used for isolation and/or ID of fungi
Discuss clinical significance of organisms listed in lab
List the dimorphic forms of fungi
Smooth, creamy OR fluffy, powdery
Discuss respiratory specimen collection/ handling in mycology
- collected immediately in the morning
- cough deeply from lungs (NOT SALIVA) = THICK, not watery
- sputum must arrive in lab within 2 hours of collection
What temp are respiratory mycology specimens stored if not immediately sent to lab ?
4°C in fridge
Describe blood specimen collection/ handling in mycology ?
- 70% alcohol antiseptic
- 2 bottles (aerobic and *) at 2 sites
- If dimorphic, 10 mL in SPS or isolator tube = held RT for 4 weeks
SPS - Sodium polyanethole sulfonate
Discuss tissue collection / handling in mycology
- sterile technique + gauze moistened w/ sterile saline
Discuss CSF/ sterile body fluid collection/ handling in mycology
- 2 sterile 10 mL tubes (2nd tube is submitted to micro)
- Fungal culture includes cryptococcal Ag testing + calcofluor white stain
- India ink stain used to ID large capsules seen with Cryptococcus
Discuss MSU collection/ handling in mycology
- first morning collection
- ensure proper disinfection to remove normal flora
- urine into toilet before and after collection
- grey top vacutainer is okay at RT; 4°C in fridge if >20 min
Discuss hair specimen collection/ handling in mycology
- fungal collection kit (envelope, black paper)
- forcep >10 hair (uncut)
- clean scalp not required
Hx:
- anti-fungal therapy
- travel
- predisposing factors (animal contact, immunodeficiency)
Discuss skin specimen collection/ handling in mycology
- fungal collection kit (envelope, black pepper)
- disinfect with 70% alcohol
- scrape with sterile scalpel
Hx:
- anti-fungal therapy
- travel
- predisposing factors (animal contact, immunodeficiency)
Discuss nail specimen collection/ handling in mycology
- fungal collection kit (envelope, black paper)
- clean w/ 70% alcohol
- use scalpel to remove debris from under nail
Hx: - anti-fungal therapy
- travel
- predisposing factors (animal contact, immunodeficiency)
SDA use and selective ingredients
Sabouraud Dextrose Agar
Use: ENRICHED; primary recovery of saprobes (including dermatophytes)
Ingredients: sabouraud dextrose + brain-heart infusion agar