Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BSMLS2A FINALS > MLSBIO101 RECOMBINANT DNA TECHNOLOGY > Flashcards

MLSBIO101 RECOMBINANT DNA TECHNOLOGY Flashcards

1
Q

What do you call DNA molecules that are extracted from different sources and chemically joined together?

A

Recombinant DNA Technology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the DNA comprising animal gene which may be recombined with DNA from a bacterium?

A

Recombinant DNA Technology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What year is the discovery of DNA structure by Watson and Crick?

A

1953

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Who discovered DNA structure in 1953?

A

Watson and Crick

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What year is the isolation of DNA ligase?

A

1967

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What year is the isolation of REase?

A

1970

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What year did Paul Berg generated rDNA technology?

A

1972

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Who generated rDNA technology in 1972?

A

Paul Berg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What year did Cohen and Boyer produced the first plasmid vector capable of being replicated within a bacterial host?

A

1973

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Who produced the first plasmid vector capable of being replicated within a bacterial host?

A

Cohen and Boyer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the goals of recombinant DNA technology?

A
  1. To isolate and characterize a gene
  2. TO make desired alterations in one or more isolated genes
  3. To return altered genes to living cells
  4. Artificially synthesize new gene
  5. Alternating the genome of an organism
  6. Understanding the hereditary diseases and their cure
  7. Improving human genome
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are the 4 procedures of making rDNA?

A
  1. Isolating of DNA
  2. Cutting of DNA
  3. Joining of DNA
  4. Amplifying of DNA
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

In Isolation of DNA, cells are lysed using ____ in order to disrupt the plasma membrane.

A

Detergent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

In Isolation of DNA, after lysing the cells, it is treated with____ to destroy protein and ___ to destroy RNA.

A

Protease and RNAase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

In isolation of DNA, after centrifuging the test tube, what do you transfer to a clean tube?

A

Supernatant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

In Isolation of DNA, What do you use to precipitate the DNA after transferring the supernatant to a clean test tube?

A

Ethanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

In cutting of DNA, how does DNA cut into large fragments?

A

Mechanical Shearing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

In cutting of DNA, what are the scissors of molecular genetics?

A

Restriction Enzymes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is a special class of sequence-specific enzyme?

A

Restriction Enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What enzyme is found in bacteria?

A

Restriction Enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

T/F. Site-specific-cleave DNA molecules only cleaves at specific nucleotide sequence.

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What characteristic do REases recognize DNA base sequence?

A

Palindrome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

T/F. REase make staggered cuts with complementary base sequences for easy circulization.

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

In amplifying the recombinant DNA, To what is the recombinant DNA transformed into?

A

Bacterial host strain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

In Amplification, what do you treat the cells?

A

CaCl2 or Calcium chloride

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

In amplification, what temperature are the cells heat shocked?

A

42 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

What enzyme binds to DNA molecules?

A

DNA Ligase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

What enzyme cleaves DNA at specific sites?

A

Type II Restriction Endonuclease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

What enzyme makes a DNA copy of RNA molecule?

A

Reverse Transcriptase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

What enzyme fills single stranded gapes of DNA duplex?

A

DNA Polymerase I

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

What enzyme adds phosphate to the 5’-OH end of a polynucleotide?

A

Polynucleotide Kinase

32
Q

What enzyme adds homopolymer tails to the 3’-OH ends?

A

Terminal Transferase

33
Q

What enzyme removes nucleotide residues from 3’ ends?

A

Exonuclease III

34
Q

What enzyme removes nucleotides from the 5’ ends?

A

Bacteriophage {Lamda} Exonuclease

35
Q

What enzyme removes terminal phosphates?

A

Alkaline Phosphatase

36
Q

What do you call the area of DNA that can join another DNA part without losing the limit for self-replication?

A

Vector

37
Q

T/F. A vector does not lose the limit for self-replication.

A

True

38
Q

What is capable of replicating in host cell?

A

Vector

39
Q

T/F. A vector should have convenient RE sites for inserting DNA of interest.

A

True

40
Q

T/F. A vector should have a selectable marker to indicate which host cells received recombinant DNA molecule?

A

True

41
Q

T/F. A vector should be small and easy to isolate.

A

True

42
Q

What do you call the small, circular DNA molecules that are separated from the rest of the chromosome?

A

Plasmids

43
Q

What vector replicates independently of the bacterial chromosome?

A

Plasmids Vector

44
Q

What vector is useful for cloning DNA inserts less than 20 kb (kilobase pairs)?

A

Plasmids Vector

45
Q

What vector is the recombinant infections, containing the phage chromosome in addition to embedded “outside” DNA?

A

Lamba Phage Vectors

46
Q

T/F. Phage vectors can convey bigger DNA groupings than plasmid vectors.

A

True

47
Q

What vector is the hybrids of phages and plasmids that can carry DNA fragments up to 45kb?

A

Cosmids

48
Q

What vector can replicate like plasmids but can package like phage lambda?

A

Cosmids

49
Q

What are the vectors that carry host signals that facilitate the transcription and translation of an inserted gene?

A

Expression Vectors

50
Q

What vector is useful for expressing eukaryotic genes in bacteria?

A

Expression Vectors

51
Q

What vector has been engineered to contain a centromere, telomere, origin of replication, and a selectable marker?

A

Yeast Artificial Chromosome (YACS)

52
Q

What vector can carry up to 1000 kb of DNA?

A

Yeast Artificial Chromosomes (YACS)

53
Q

What vector is useful for cloning eukaryotic genes that contains introns?

A

Yeast Artificial Chromosomes (YACS)

54
Q

What vector are bacterial plasmids derived from the F plasmid?

A

Bacterial Artificial chromosomes (BACS)

55
Q

What vector is capable of carrying up to 300kb of DNA?

A

Bacterial Artificial Chromosomes (BACS)

56
Q

What are the techniques used in rDNA technology?

A
  1. Gel Electrophoresis
  2. Cloning Libraries
  3. Restriction Enzyme Mapping
  4. PCR
  5. Nucleic Acid Hybridization
  6. DNA Microarrays
57
Q

What technique are DNA fragments of different sizes can be separated by an electrical field applied to a gel?

A

Gel Electrophoresis

58
Q

What technique does the negatively charged DNA migrates away from the negative electrode and to the positive electrode?

A

Gel Electrophoresis

59
Q

T/F. In gel electrophoresis, the smaller the fragment, the faster it migrates.

A

True

60
Q

What do you call the collection of DNA clones in a certain vector?

A

Libraries

61
Q

T/F. The goal of cloning libraries is to have each gene representing in the library at least once.

A

True

62
Q

What is made from RE DNA fragments of total genomic DNA?

A

Genomic

63
Q

What is made from DNA synthesized from mRNA?

A

cDNA (Complementary DNA)

64
Q

What technique allows the isolation of specific segment of DNA from a small DNA (or cell sample) using DNA primers at the ends of the segment of interest?

A

PCR

65
Q

T/F. It is important to have a restriction enzyme site map of a cloned gene for further manipulations of the gene.

A

True

66
Q

What is accomplished by digestion of the gene singly with several enzymes and then in combinations?

A

Restriction Enzyme Mapping

67
Q

T/F. In restriction enzyme mapping, the fragments are subjected to gel electrophoresis to separate the fragments by size and the sites are deduced based on the sizes of the fragments.

A

True

68
Q

In Nucleic Acid Hybridization, what blot allows the detection of a gene of interest by probing DNA fragments that have been separated by electrophoresis with a “labeled” probe?

A

Southern Blot

69
Q

In Nucleic Acid Hybridization, what blot probe RNA on a gel with DNA probe?

A

Northern Blot

70
Q

In Nucleic Acid Hybridization, what blot probes proteins on a gel with an antibody?

A

Western Blot

71
Q

What technique is the vast majority of the protein-encoding qualities onto a microarray chip, utilizing innovation in light of the DNA silicon chip industry?

A

DNA Microarrays

72
Q

In DNA Microarrays, what is utilized to hybridize to cell RNA, and measure the statement rates of a substantial number of qualities in a cell?

A

Chip

73
Q

To what are applies the growing crops of your choice (GM food), pesticide resistant crops, fruits with attractive colors, all being grown in artificial conditions?

A

Agriculture

74
Q

To what area applies where artificial insulin production, drug delivery to target sites?

A

Pharmacology

75
Q

To what area applies gene therapy, antiviral therapy, vaccination, and synthesizing clotting factors?

A

Medicine

BSMLS2A FINALS (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions