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Diagnostic Testing > Mixing and Diluting > Flashcards

Mixing and Diluting Flashcards

1
Q

Types of extract

A
  • Aqueous
  • Freeze Dried
  • Alum-Precipitated
  • Modified Not used in the U. S.
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2
Q

HSA is used as a diluent for what allergen extracts?

A
  • Aqueous extracts

- Freeze-Dried Extracts

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3
Q

Phenol Saline is used as a diluent for what type of allergen extracts.

A

Alum-Precipated

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4
Q

What factors affect the stability of extracts?

A
  • Temperature
  • Dilution
  • Diluent
  • Vial Volume
  • Proteolytic Enzymes
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5
Q

V/V

A

Volume/Volume

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6
Q

W/V

A

Weight/Volume

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7
Q

PNU

A

Protein Nitrogen Unit

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8
Q

AU

A

Allergy Unit

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9
Q

BAU

A

Bioequivalent Allergy Unit

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10
Q

What are the two types of flow hoods?

A
  • Horizontal Flow Hood

- Vertical Flow Hood

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11
Q

What are your source materials for allergen extracts?

A
  • Pollens
  • Molds
  • Insects
  • Foods
  • Dust
  • Mites
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12
Q

What are the minimum requirements for a label?

A
  • Patients Name
  • Concentration
  • Contents
  • Number/Color Code
  • Expiration Date
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13
Q

T

A

Trees

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14
Q

G

A

Grass

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15
Q

C

A

Cat

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16
Q

HD

A

Dust, House

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17
Q

Dmf

A

Mites, farinae

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18
Q

Cr

A

Cockroach

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19
Q

Ge

A

Gerbil

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20
Q

Ha

A

Hamster

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21
Q

Mo

A

Mouse

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22
Q

Rt

A

Rat

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23
Q

W

A

Weeds

24
Q

M

A

Molds

25
Q

D

A

Dog

26
Q

Fmx

A

Feather, mix

27
Q

Dmp

A

Mites, pteronyssinus

28
Q

Dmx

A

Mites mix

29
Q

Gp

A

Guinea Pig

30
Q

H

A

Horse

31
Q

Rb

A

Rabbit

32
Q

Reasons for making a 10 fold dilution

A
  • Dropped vial
  • Vial expired
  • Venom Allergy Skin Testing
  • Delayed testing using source antigen
33
Q

How many vial volumes are available?

A

3

34
Q

What are the vial volumes available?

A
  • 10ml
  • 5ml
  • 2ml
35
Q

Vial 1
Color:
Concentration:

A

Red

1:1v/v

36
Q

Vial 2
Color:
Concentration:

A

Yellow

1:10 v/v

37
Q

Vial 3
Color:
Concentration:

A

Blue

1:100v/v

38
Q

Vial 4
Color:
Concentration:

A

Green

1/1000 v/v

39
Q

Vial 5
Color:
Concentration:

A

Silver

1:10000 v/v

40
Q

When does vial 1 and vial 2 expire?

A

12 months from the date mixed, not to exceed source vial expiration date.

41
Q

When does vial 3, vial 4 and vial 5 expire?

A

6 months from the date mixed, not to exceed source vial expiration date.

42
Q

A 5ml vial comes pre-filled with how much diluent?

A

4.5ml diluent

43
Q

A 2ml vial comes pre-filled with how much diluent?

A

1.8ml diluent

44
Q

A 10ml vial comes pre-filled with how much diluent?

A

9ml diluent

45
Q

List the vials in order from weakest to strongest.

A 
Silver
Green 
Blue
Yellow
Red
46
Q

Why are extracts sent freeze dried?

A
  • Technicians are trained to make 10 fold dilutions
  • Must be refrigerated after reconstitution
  • Short expiration dates
47
Q

When do prick testing materials expire?

A

12 months

48
Q

When do intradermal testing materials expire?

A

6 months

49
Q

When do anergy panel testing materials expire?

A

6 months

50
Q

When does the red vial expire?

A

12 months

51
Q

When does the yellow vial expire?

A

12 months

52
Q

When does the blue vial expire?

A

6 months

53
Q

When does the green vial expire?

A

6 months

54
Q

When does the silver vial expire?

A

6 months

55
Q

When do venom freeze dried products expire?

A

Manufacturer expiration date

Diagnostic Testing (8 decks)

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