Mixing and Diluting Flashcards
1
Q
Types of extract
A
- Aqueous
- Freeze Dried
- Alum-Precipitated
- Modified Not used in the U. S.
2
Q
HSA is used as a diluent for what allergen extracts?
A
- Aqueous extracts
- Freeze-Dried Extracts
3
Q
Phenol Saline is used as a diluent for what type of allergen extracts.
A
Alum-Precipated
4
Q
What factors affect the stability of extracts?
A
- Temperature
- Dilution
- Diluent
- Vial Volume
- Proteolytic Enzymes
5
Q
V/V
A
Volume/Volume
6
Q
W/V
A
Weight/Volume
7
Q
PNU
A
Protein Nitrogen Unit
8
Q
AU
A
Allergy Unit
9
Q
BAU
A
Bioequivalent Allergy Unit
10
Q
What are the two types of flow hoods?
A
- Horizontal Flow Hood
- Vertical Flow Hood
11
Q
What are your source materials for allergen extracts?
A
- Pollens
- Molds
- Insects
- Foods
- Dust
- Mites
12
Q
What are the minimum requirements for a label?
A
- Patients Name
- Concentration
- Contents
- Number/Color Code
- Expiration Date
13
Q
T
A
Trees
14
Q
G
A
Grass
15
Q
C
A
Cat
16
Q
HD
A
Dust, House
17
Q
Dmf
A
Mites, farinae
18
Q
Cr
A
Cockroach
19
Q
Ge
A
Gerbil
20
Q
Ha
A
Hamster
21
Q
Mo
A
Mouse
22
Q
Rt
A
Rat
23
Q
W
A
Weeds
24
Q
M
A
Molds
25
D
Dog
26
Fmx
Feather, mix
27
Dmp
Mites, pteronyssinus
28
Dmx
Mites mix
29
Gp
Guinea Pig
30
H
Horse
31
Rb
Rabbit
32
Reasons for making a 10 fold dilution
- Dropped vial
- Vial expired
- Venom Allergy Skin Testing
- Delayed testing using source antigen
33
How many vial volumes are available?
3
34
What are the vial volumes available?
- 10ml
- 5ml
- 2ml
35
Vial 1
Color:
Concentration:
Red
| 1:1v/v
36
Vial 2
Color:
Concentration:
Yellow
| 1:10 v/v
37
Vial 3
Color:
Concentration:
Blue
| 1:100v/v
38
Vial 4
Color:
Concentration:
Green
| 1/1000 v/v
39
Vial 5
Color:
Concentration:
Silver
| 1:10000 v/v
40
When does vial 1 and vial 2 expire?
12 months from the date mixed, not to exceed source vial expiration date.
41
When does vial 3, vial 4 and vial 5 expire?
6 months from the date mixed, not to exceed source vial expiration date.
42
A 5ml vial comes pre-filled with how much diluent?
4.5ml diluent
43
A 2ml vial comes pre-filled with how much diluent?
1.8ml diluent
44
A 10ml vial comes pre-filled with how much diluent?
9ml diluent
45
List the vials in order from weakest to strongest.
```
Silver
Green
Blue
Yellow
Red
```
46
Why are extracts sent freeze dried?
- Technicians are trained to make 10 fold dilutions
- Must be refrigerated after reconstitution
- Short expiration dates
47
When do prick testing materials expire?
12 months
48
When do intradermal testing materials expire?
6 months
49
When do anergy panel testing materials expire?
6 months
50
When does the red vial expire?
12 months
51
When does the yellow vial expire?
12 months
52
When does the blue vial expire?
6 months
53
When does the green vial expire?
6 months
54
When does the silver vial expire?
6 months
55
When do venom freeze dried products expire?
Manufacturer expiration date
