Mixing and Diluting

Question 1

Types of extract

Answer 1

• Aqueous
• Freeze Dried
• Alum-Precipitated
• Modified Not used in the U. S.

Question 2

HSA is used as a diluent for what allergen extracts?

Answer 2

• Aqueous extracts

- Freeze-Dried Extracts

Question 3

Phenol Saline is used as a diluent for what type of allergen extracts.

Answer 3

Alum-Precipated

Question 4

What factors affect the stability of extracts?

Answer 4

• Temperature
• Dilution
• Diluent
• Vial Volume
• Proteolytic Enzymes

Question 5

V/V

Answer 5

Volume/Volume

Question 6

W/V

Answer 6

Weight/Volume

Question 7

PNU

Answer 7

Protein Nitrogen Unit

Question 8

AU

Answer 8

Allergy Unit

Question 9

BAU

Answer 9

Bioequivalent Allergy Unit

Question 10

What are the two types of flow hoods?

Answer 10

• Horizontal Flow Hood

- Vertical Flow Hood

Question 11

What are your source materials for allergen extracts?

Answer 11

• Pollens
• Molds
• Insects
• Foods
• Dust
• Mites

Question 12

What are the minimum requirements for a label?

Answer 12

• Patients Name
• Concentration
• Contents
• Number/Color Code
• Expiration Date

Question 13

T

Answer 13

Trees

Question 14

G

Answer 14

Grass

Question 15

C

Answer 15

Cat

Question 16

HD

Answer 16

Dust, House

Question 17

Dmf

Answer 17

Mites, farinae

Question 18

Cr

Answer 18

Cockroach

Question 19

Ge

Answer 19

Gerbil

Question 20

Ha

Answer 20

Hamster

Question 21

Mo

Answer 21

Mouse

Question 22

Rt

Answer 22

Rat

Question 23

W

Answer 23

Weeds

Question 24

M

Answer 24

Molds

Question 25

D

Answer 25

Dog

Question 26

Fmx

Answer 26

Feather, mix

Question 27

Dmp

Answer 27

Mites, pteronyssinus

Question 28

Dmx

Answer 28

Mites mix

Question 29

Gp

Answer 29

Guinea Pig

Question 30

H

Answer 30

Horse

Question 31

Rb

Answer 31

Rabbit

Question 32

Reasons for making a 10 fold dilution

Answer 32

• Dropped vial
• Vial expired
• Venom Allergy Skin Testing
• Delayed testing using source antigen

Question 33

How many vial volumes are available?

Answer 33

3

Question 34

What are the vial volumes available?

Answer 34

• 10ml
• 5ml
• 2ml

Question 35

Vial 1
Color:
Concentration:

Answer 35

Red

1:1v/v

Question 36

Vial 2
Color:
Concentration:

Answer 36

Yellow

1:10 v/v

Question 37

Vial 3
Color:
Concentration:

Answer 37

Blue

1:100v/v

Question 38

Vial 4
Color:
Concentration:

Answer 38

Green

1/1000 v/v

Question 39

Vial 5
Color:
Concentration:

Answer 39

Silver

1:10000 v/v

Question 40

When does vial 1 and vial 2 expire?

Answer 40

12 months from the date mixed, not to exceed source vial expiration date.

Question 41

When does vial 3, vial 4 and vial 5 expire?

Answer 41

6 months from the date mixed, not to exceed source vial expiration date.

Question 42

A 5ml vial comes pre-filled with how much diluent?

Answer 42

4.5ml diluent

Question 43

A 2ml vial comes pre-filled with how much diluent?

Answer 43

1.8ml diluent

Question 44

A 10ml vial comes pre-filled with how much diluent?

Answer 44

9ml diluent

Question 45

List the vials in order from weakest to strongest.

Answer 45

Silver
Green 
Blue
Yellow
Red

Question 46

Why are extracts sent freeze dried?

Answer 46

• Technicians are trained to make 10 fold dilutions
• Must be refrigerated after reconstitution
• Short expiration dates

Question 47

When do prick testing materials expire?

Answer 47

12 months

Question 48

When do intradermal testing materials expire?

Answer 48

6 months

Question 49

When do anergy panel testing materials expire?

Answer 49

6 months

Question 50

When does the red vial expire?

Answer 50

12 months

Question 51

When does the yellow vial expire?

Answer 51

12 months

Question 52

When does the blue vial expire?

Answer 52

6 months

Question 53

When does the green vial expire?

Answer 53

6 months

Question 54

When does the silver vial expire?

Answer 54

6 months

Question 55

When do venom freeze dried products expire?

Answer 55

Manufacturer expiration date