Mitosis/Bacterial & Viral RP Flashcards
What are chromosomes joined in the middle by?
A centromere.
What is a sister chromatid?
Two strands on the same chromosome.
What are centrioles?
Proteins which move to opposite ends of the cell and produce spindle fibres.
What don’t plants have regarding prophase?
Centrioles or spindle fibres.
What is binary fission?
A form of mitosis, the cell replicates its genetic material before splitting into 2 DC.
What do we grow colonies of bacteria on to observe BF?
Agar gel plates or in nutrient broth.
Why do we grow cultures of microorganisms?
To investigate the action of disinfectants and antibiotics.
What’s the difference between circular DNA and plasmids?
ODNA replicates once but plasmids can do many times.
How does a circular DNA replicate?
Cell englarges, circular moves to opposite poles of the cell & attach to the cell membrane.
Cytoplasm begins to divide and a new cell wall forms.
CP splits, two DC.
What are viruses?
Nucleic acids surrounded by protein.
What are 4 properties of viruses?
-Can only reproduce inside host cells.
-No cell surface membrane, RS and CP.
-Protein coat called a capsid, with attachment proteins sticking out.
-Attachment proteins bind to the host cell.
Why do different viruses require different receptor proteins?
They have different attachment proteins.
How do viruses replicate?
-Inject their DNA into a host cell.
-Incorporate their DNA into the host cell’s DNA.
-They then use the host cell’s reproductive DNA code to replicate nucleic acids, enzymes + structural proteins which are assembled into new viruses.
describe the events of prophase
chromosomes condense to become visible and fat
centrioles split and move to opposite ends of the cell, spanning spindle fibres from pole to pole to produce the spindle apparatus
the nuclear envelope disintegrates and chromosomes are freed into the cytoplasm
describe the events that occur in metaphase
spindle fibres attach to the chromosomes at their centromeres and chromosomes are pulled along the apparatus to line up along the equator of the cell