Misc. Testing Flashcards
How can we remove antibodies from serum?
Adding the target antigen
Antibody binds to antigen
What is used to absorb Bg-like antibodies from serum?
Human Platelet Concentrate
What does RESt (Rabbit Erythrocyte Stroma) posess?
I
H
IH-like structures
What is Homologous Adsorption?
Patient is so anemic that they do not have enough autologous RBC’s to perform adsorptions
What is the simplest method of Autoadsorption?
Using the patients own RBC’s
What are the first three steps of Autoadsorption?
Wash
Treat
Incubate Cells up to ONE HOUR
What can cause phenotyping a patient to be difficult?
Positive DAT
Recent Transfusion
Differential Adsorption to be performed
What are Elution techniques used for?
Release, Concentrate, and Purify antibodies
What is the term given to antibody release and total destruction of RBC antigens?
Total Elution
What is the term given to antibody removal but RBC antigens remain intact?
Partial Elution
What are Chloroquine diphosphate, EGA, and ZZAP used for?
Reagents in partial elution
What are the temperature dependent methods?
Gentle Heat (45C)
Total Elution (56C)
Lui Freeze (-18C)
pH (Wash, 3 pH, neutralized)
What are Dichloromethane, Xylene, and Ether used for?
Used for total elution (organic solvents)
Reverse Van der Waals forces
Very potent
Why do we make dilutions?
To make strong solutions weaker
What is the definition of a “High titer”?
High concentration of antibodies in the serum
What do we test a Twofold Serial Dilution against?
Suspension of RBC’s that posses the target antigen
What can lead to falsely elevated titer level results?
Contamination from a higher titer tube
