Antiglobulin Test

Question 1

What did the use of AHG tests lead to the discovery of?

Answer 1

Kell
Duffy
Kidd

Question 2

What can polyspecific AHG reagent be sensitized with?

Answer 2

IgG
C3d
Or both

Question 3

What is an advantage of using monoclonal AHG?

Answer 3

Higher titer of Ab than polyclonal

Question 4

What is the principle of IAT (Indirect Antiglobulin Testing) testing?

Answer 4

Detect in-vitro sensitized red cells
Two stage procedure

Question 5

What is the principle of DAT (Direct Antiglobulin Testing) testing?

Answer 5

Detect in-vivo sensitized red cells with IgG

Question 6

What does DAT not require?

Answer 6

Incubation phase

Question 7

What can detect between 100 and 200 IgG or C3?

Answer 7

IAT (Indirect Antiglobulin Testing)

Question 7

What can detect IgG at levels of 100-500 and C3d at 400-1100?

Answer 7

DAT (Direct Antiglobulin Testing)

Question 8

What can influence the sensitization rate of IgG molecules on RBCs?

Answer 8

Washing of RBC’s
Centrifugation

Question 9

What can lead to a false positive due to T-activation of cells?

Answer 9

Bacterial Contamination