Mil bio lab quiz 3

Question 1

is a nucleic acid amplification technique (NAAT). In diagnostic clinical laboratories, this technique is primarily used for the following purposes:

Answer 1

polymerase chain reaction (PCR)

Question 2

Contains the target region of interest

Answer 2

DNA template

Question 3

Oligonucleotides that flank the region of interest

Answer 3

Primer pair

Question 4

Locates the replication start point along the template’s sense strand

Answer 4

Forward

Question 5

Marks a second replication start point along the antisense, downstream of the forward primer

Answer 5

Reverse

Question 6

High heat to disrupt and separates DNA duplexes

Answer 6

Heat denaturation:

Question 7

Such as Taq polymerase, to catalyze DNA-dependent DNA synthesis

Answer 7

Thermostable polymerase

Question 8

Primer-template hybridization at 3-5°C belo

Answer 8

Primer annealing

Question 9

Optimal temperature for polymerase activity

Answer 9

Primer extension:

Question 10

Full dissociation of template DNA duplexes

Answer 10

First denaturation:

Question 11

Repeated cycling of the PCR thermal steps to double DNA per cycle, allowing exponential amplification of the target DNA

Answer 11

Thermal cycling

Question 12

This phase is also known as the geometric amplification phase, where efficiency of PCR amplification is ~100 % (i.e. each cycle results in double the amplified nucleic, as theorized)

Answer 12

Exponential amplification phase

Question 13

Finish replication on all created DNA templates

Answer 13

Final extension