Bacte lect Flashcards
Facultative anaerobe
Isolation Media: SBA, MSA, CAN. PEA agar CATALASE (+) __
STAPHYLOCOCCI
Enterotoxins A – E Enterotoxins G, J
COAGULASE
main virulence factor
a. Bound coagulase/Clumping Factor
b. Free Coagulase
causes bacteria to agglutinate in plasma
definitive test for S. aureus; stand alone test; to differentiate S. aureus from other staphylococci
COAGULASE TEST
S. aureus produces
Beta-lactamase
To determine if S. aureus considered as the GOLD STANDARD
Polymerase Chain Reaction (PCR) molecular testing
Bacillus anthracis a.k.a ANTHRAX BACILLUS
Non-motile
Media to isolate Bacillus anthracis a.k.a ANTHRAX BACILLUS
5% SBA
Culture media Bacillus cereus a.k.a. Fried rice Syndrome
5% SBA - isolation media
H2S positive on TSI media, non-motile and catalase and oxidase negative;
Erysipelothrix rhusiopathiae
What is the positive result of biochemical test NIACIN TEST
Yellow
What is the positive result of biochemical test TWEEN 80 HYDROLYSIS TEST
Pink
Red / Red,
K/K, no sugar fermented, non fermenters
(B and C answer)
CHOLERA LIKE
ENTEROTOXIGENIC E. COLI ETEC
bloody diarrhea
ENTEROINVASIVE E. COLI EIEC
Non-toxigenic and non-invasive but can cause diarrhea in infants / infantile diarrhea
E. coli serotype 0111
ENTEROPATHOGENIC E. COLI EPEC
Can cause acute & persistent diarrhea in children and adults
ENTEROAGGREGATIVE E. COLI EAEC
This constitutes the 6th group in the diarrheagenic E. coli.
Can cause diarrhea in otherwise healthy patrients
DIFFUSELY ADHERENT E. COLI DAEC
Can cause SEVERE BLOOD DIARRHEA due to its ability to produce VEROTOXIN HUS -hemolytic uremic syndrome, Most severe manifestation of EHEC
Specific example is E. coli 0157:H7 (Mug Test - Negative)
} persistent pediatric diarrhea
eating poorly cooked meal like hamburger
ENTEROHEMORRHAGIC E. COLI VEROTOXIC E. COLI SHIGA TOXIN PRODUCING
Can cause secretory diarrhea in AIDS patients with Inflammatory bowel disease Oxidase (+)
Plesiomonas shiglloides
Formerly called Campylobacter coli; habitat: human gastric mucosa
Helicobacter pylori
Also known as hemin or hematin
X factor
Media: BCYE- buffer charcoal yeast extract
Genus LEGIONELLA
A common laboratory test for Legionnaire’s disease
Urinary antigen test
Rapid test to detect Brucellosis
Serum agglutination
