Bacte lect Flashcards
Facultative anaerobe
Isolation Media: SBA, MSA, CAN. PEA agar CATALASE (+) __
STAPHYLOCOCCI
Enterotoxins A – E Enterotoxins G, J
COAGULASE
main virulence factor
a. Bound coagulase/Clumping Factor
b. Free Coagulase
causes bacteria to agglutinate in plasma
definitive test for S. aureus; stand alone test; to differentiate S. aureus from other staphylococci
COAGULASE TEST
S. aureus produces
Beta-lactamase
To determine if S. aureus considered as the GOLD STANDARD
Polymerase Chain Reaction (PCR) molecular testing
Bacillus anthracis a.k.a ANTHRAX BACILLUS
Non-motile
Media to isolate Bacillus anthracis a.k.a ANTHRAX BACILLUS
5% SBA
Culture media Bacillus cereus a.k.a. Fried rice Syndrome
5% SBA - isolation media
H2S positive on TSI media, non-motile and catalase and oxidase negative;
Erysipelothrix rhusiopathiae
What is the positive result of biochemical test NIACIN TEST
Yellow
What is the positive result of biochemical test TWEEN 80 HYDROLYSIS TEST
Pink
Red / Red,
K/K, no sugar fermented, non fermenters
(B and C answer)
CHOLERA LIKE
ENTEROTOXIGENIC E. COLI ETEC
bloody diarrhea
ENTEROINVASIVE E. COLI EIEC
Non-toxigenic and non-invasive but can cause diarrhea in infants / infantile diarrhea
E. coli serotype 0111
ENTEROPATHOGENIC E. COLI EPEC
Can cause acute & persistent diarrhea in children and adults
ENTEROAGGREGATIVE E. COLI EAEC
This constitutes the 6th group in the diarrheagenic E. coli.
Can cause diarrhea in otherwise healthy patrients
DIFFUSELY ADHERENT E. COLI DAEC
Can cause SEVERE BLOOD DIARRHEA due to its ability to produce VEROTOXIN HUS -hemolytic uremic syndrome, Most severe manifestation of EHEC
Specific example is E. coli 0157:H7 (Mug Test - Negative)
} persistent pediatric diarrhea
eating poorly cooked meal like hamburger
ENTEROHEMORRHAGIC E. COLI VEROTOXIC E. COLI SHIGA TOXIN PRODUCING
Can cause secretory diarrhea in AIDS patients with Inflammatory bowel disease Oxidase (+)
Plesiomonas shiglloides
Formerly called Campylobacter coli; habitat: human gastric mucosa
Helicobacter pylori
Also known as hemin or hematin
X factor
Media: BCYE- buffer charcoal yeast extract
Genus LEGIONELLA
A common laboratory test for Legionnaire’s disease
Urinary antigen test
Rapid test to detect Brucellosis
Serum agglutination
Test for Beta Lactamase Production
Cephalosporinase test
1 cause of SBE sub acute bacterial endocarditis
Viridans streptococci
1 cause of neonal meningitis
Streptococcus agalactiae
Virulence Factors of S. pyogenes
None
Causes food poisoning
All of these
flaccid paralysis
Clostridium botulinum
MODIFIED ELEK’S in vitro test
E
Paps stain smear
Gardnella vaginalis
Digestion and decontamination
Mycobacterium detection
TWEEN 80 HYDROLYSIS TEST
M. kansasii
All Genus VIBRIO oxidase + except
V. metschnikovii
Media cetrimide used for
Pseudomonas aeruginosa
Causes so called human bite wound
Cardiobacterium hominis
Mercury droplets/ shiny white pearls
Bordetella pertussis
Media: BCGA - blood cystine glucose agar
Genus Francisella
like a coin with a raised rim,
Streptococcus pneumoniae
K/K
Negative: deA, Positive: deCar
Enzyme produce by lactose fermenters
Beta galactosidase
Lactose permease
Media for e coli 157
Mac conkey sorbitol A
Bulls eye rash of lyme disease
Ecm Erythema chronicum migrans
Blue pigment of p. Aeruginosa
Pyocyanin
Bone shape dog biscuit
Bifidobacterium
Media for f. Tularensis
Blood cystine glucose agar
Flaccid paralysis
Clostridium botulinum
Diagnostic form of g vaginalis on paps stain smear
Clue cells
Clenched fist wound
Eikenella corrodens
Bordetella pertusis
Aerobe
Mycobacterium
Aerobe
Vibrio
F. Anaerobe
M. Pylori
Microaero
E. Alactolyticum
Anaerobe
Antibiotic for anaerobes except: Vanco kana colistin
Nystatin
Pplo group=
Mycoplasma
Helicobacter рузогі
Microaerophilic
Elementary & reticulated body
Chlamydia
Corn meal agar
M. Xenopt
Butcher’s cut
Erysipelothrix rhusio
Slighty acid fast, fresnly flowed field, TB like
Nocardia
BrickRed in UV
Prevotella and Poly
Yellow in TCBS
Vibrio
Staphylococci
Facultative anaerobe
Enterobacteriaceae
Facultative anaerobe
Mycoplasma
Facultative anaerobe
Secondary syphilis
Extra Genital Lesions
B. Petussis
aerobe
H. Influenzae
facultative anaerobe
M. Tuberculosis
obligate aerobe
Bovis
Aerobe
Rapid test for helicobacter pylori
Copro stool antigen test
Common lab test for l. Pneumophilia
Urinary antigen test
Greening of agar exposure to air
Fusobacterium
LIA result of salmonella
K/K H2S(+) Decar (+) Dea (-)
T strain mycoplasma
Ureaplasma urealyticum
H2S (+) gram (-)
P.vulgaris, mirabilis, citrobacter freundii, edwardsiella tarda, sal
Positive result bile solubility
Lysis of colonies / clearing S.pneumonaie
Black on CTBA-
Corynebacterium diptheriae
Slightly acid fast, freshly flowed field, TB like
Nocardia
Not zoonosis
Legionella