Midterms (gram positive) Flashcards
Catalase Positive
Staphylococcus
Catalase Negative
Streptococcus
Enzyme that able to decompose hydrogen peroxide into water and oxygen
Catalase Test
Creates clot
Coagulase test
Coagulase positive
Staphylococcus aureus
Coagulase negative
Staphylococcus saprophyticus
Staphylococcus epidermidis
An enzyme that allows the conversion of fibrinogen to fibrin
Coagulase
Cause clotting
Fibrin
An antibacterial agent
Novobiocin Test
Zone where there’s no growth
Zone of Inhibition
Novobiocin-resistant
Zone of inhibition is less than 16 mm
Novobiocin-sensitive
Zone of inhibition is greater than 6 mm
Novobiocin resistant
Staphylococcus saprophyticus
Novobiocin sensitive
Staphylococcus epidermidis
Differential Media
Mannitol Salt Agar
Culture media where we can differentiate family and species
Mannitol Salt Agar
Presence of Golden yellow pigment indicates the presence of
Staphylococcus aureus
Porcelain white pigment
Staphylococcus epidermidis
No pigment
Staphylococcus saprophyticus
Staphylococcus
Round shape bacteria that are in CLUSTERS “grape like”
Staphylococcus aureus
Virulence Factors
Protein A
Coagulase
Hemolysin
Leukocidins
Penicillinase
aka beta-lactamase
Penicillinase
enzyme that allows S. aureus to resist the antibacterial property of PCN or beta-lactam antibiotics
Penicillinase
MRSA
Methicillin Resistant Staphylococcus Aureus