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micro lab > Microscopy > Flashcards

Microscopy Flashcards

1
Q

A system of accurately ground lenses
arranged to give sharp, clear, magnified
images of minute objects.

A

Microscope

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2
Q

technology of making very small
things visible to the naked eye

A

Microscopy

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3
Q

Inventor of the first
compound microscope in 1608.

A

Zacharias Janssen

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4
Q

What year did Zacharias Janssen invented the first compound microscope?

A

1608

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5
Q

What are the Lenses, Objective, and how long it is to be held by hand?

A

▪ Lenses: Bi-convex
▪ Objective: Plano-convex
▪ 2.5 ft long,

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6
Q

the process of enlarging
something only in appearance. related to
scaling up visuals or images to be able to see
more detail.

A

Magnification

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7
Q

the ability of the lenses to distinguish fine detail and structure ability to distinguish two points a specified distance apart

A

Resolving Power

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8
Q

when two substances have
a different index of refraction, the light will
bend as it passes from one material to
another

A

Refractive Index

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9
Q

Regarded as the Father of
Microscopy

A

Anton Van Leeuwenhoek (1632-1723)

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10
Q

One lens; Leeuwenhoek

A

Simple Microscope

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11
Q

First to observe Bacteria and
Protozoa using his microscope

A

Anton Van Leeuwenhoek (1632-1723)

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12
Q

Magnifies images from 100x to
300x

A

Simple microscope

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13
Q

Series of lenses

A

Compound Microscope

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14
Q

Uses visible light to observe specimen

A

Light Microscopy

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15
Q

Specimen appears against a bright
background

A

Brightfield Microscopy

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16
Q

Use: various stained specimens

A

Brightfield Microscopy

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17
Q

Special condenser with opaque disk.
Light enters the objective lens

A

Darkfield Microscopy

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18
Q

Use: for microorganisms which cannot
be stained

A

Darkfield Microscopy

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19
Q

Uses condenser with annular
diaphragm which allows direct light to
pass through the condenser

A

Phase Contrast

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20
Q

Use: detailed examination of internal
structures; does not require staining

A

Phase Contrast

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21
Q

Uses a UV or near-UV source of
illumination

A

Fluorescence

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22
Q

Use: observation of natural
fluorescence and
immunofluorescence

A

Fluorescence

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23
Q

Uses different refractive indexes to
produce image

A

Differential Interference

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24
Q

Use: provides 3D images of samples

A

Differential Interference

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25
Q

Light source and condenser is on top,
above the stage

A

Inverted

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26
Q

Use: observation of living cells under
more natural conditions

A

Inverted

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27
Q

Beam of electrons instead of light

A

Electron Microscopy

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28
Q

Greater resolving power

A

Electron Microscopy

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29
Q

Electrons are reflected from the
specimen; magnifies 1,000 to 10,000X

A

Scanning Electron Microscope (SEM)

30
Q

Use: study of surface features of cells
and viruses

A

Scanning Electron Microscope (SEM)

31
Q

the Scanning Electron Microscope magnifies..

A

1000 to 10,000x

32
Q

Electrons pass through the specimen;
magnifies 10,000 to 100,000X

A

Transmission Electron Microscope (TEM)

33
Q

Use: examination of viruses or internal
ultrastructure in thin sections of cells

A

Transmission Electron Microscope (TEM)

34
Q

The Transmission Electron Microscope (TEM) magnifies…

A

10,000 to 100,000x

35
Q

Uses a thin metal probe that scans a
specimen

A

Scanning Tunneling Microscope (STM)

35
Q

Measures the deflection of a laser beam
aimed at the tip of a probe that travels across
the surface of the specimen

A

Atomic Force Microscopy

36
Q

Use: reveals bumps and depressions of
atoms on surface of specimen

A

Scanning Tunneling Microscope (STM)

37
Q

Use: visualization of individual
molecules and atoms

A

Atomic Force Microscopy

38
Q

Used to contain cultures of
microorganisms.

A

Screw-capped Tubes

39
Q

Used to protect substances from
contamination.

A

Screw-capped Tubes

40
Q

Used to make broth, agar deep and
agar slant.

A

Screw-capped Tubes

41
Q

Detects production of gas by
microorganisms.

A

Durham Fermentation Tube

42
Q

Simpler than Durham tube, same use.

A

Smith Fermentation Tube

43
Q

Used to make agar plates for
microbiology study.

A

Petri Dish

44
Q

Used to measure liquids to a precise
volume.

A

Volumetric Flask

45
Q

May also be used for storing liquids

A

Volumetric Flask

46
Q

Has a single graduation

A

Volumetric Pipette

47
Q

Delivers one specific volume
accurately.

A

Volumetric Pipette

48
Q

AKA blow-out pipettes

A

Serologic Pipette

49
Q

Calibrated all the way to the
tip so the last drop of the
solution will have to be blown
out of the pipette.

A

Serologic Pipette

50
Q

Has no 0 mL mark

A

Serologic Pipette

51
Q

Easier to deliver liquids with smaller
volumes with precision and accuracy.

A

Pipettor

52
Q

Tips are disposable

A

Pipettor

53
Q

Used to observe specimens for a long
time because they do not dry up very
quickly.

A

Hanging Drop Slide

54
Q

motility of an organism can be
observed.

A

Hanging Drop Slide

55
Q

AKA smear loop, inoculation wand,
microstreaker

A

Inoculating Loop

56
Q

used to retrieve and transfer
an inoculum from a culture of
microorganisms

A

Inoculating Loop

57
Q

mainly used to pick a single colony
(pure) and transfer in an agar slant
(stab and streak method)

A

Inoculating Needle

58
Q

Used to contain test tubes

A

Racks

59
Q

Uses dry heat to sterilize heat-stable
materials

A

Hot Air Sterilizer

60
Q

For sterilization of media and other
materials affected by moisture

A

Hot Air Sterilizer

61
Q

Uses moist heat or steam under
pressure

A

Autoclave

62
Q

For sterilization of media and other
materials affected by moisture

A

Autoclave

63
Q

Not sterile

A

Drying Oven

64
Q

CANNOT be used to sterilize culture
media

A

Drying Oven

65
Q

For drying specimens and lab
apparatus or glassware

A

Drying Oven

66
Q

used for growing bacterial cultures or
providing suitable conditions for a
chemical or biological reaction.

A

Incubator

67
Q

It is used in the microbiological lab for
incubations or enabling chemical
reactions to occur at higher
temperatures

A

Water Bath

68
Q

Storage for culture media

A

Refrigerator

69
Q

Preserves the morphological
characteristics of microorganisms
while arresting their growth

A

Refrigerator

micro lab (5 decks)

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