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micro lab > Microscopy > Flashcards

Microscopy Flashcards

1
Q

A system of accurately ground lenses
arranged to give sharp, clear, magnified
images of minute objects.

A

Microscope

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2
Q

technology of making very small
things visible to the naked eye

A

Microscopy

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3
Q

Inventor of the first
compound microscope in 1608.

A

Zacharias Janssen

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4
Q

What year did Zacharias Janssen invented the first compound microscope?

A

1608

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5
Q

What are the Lenses, Objective, and how long it is to be held by hand?

A

▪ Lenses: Bi-convex
▪ Objective: Plano-convex
▪ 2.5 ft long,

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6
Q

the process of enlarging
something only in appearance. related to
scaling up visuals or images to be able to see
more detail.

A

Magnification

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7
Q

the ability of the lenses to distinguish fine detail and structure ability to distinguish two points a specified distance apart

A

Resolving Power

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8
Q

when two substances have
a different index of refraction, the light will
bend as it passes from one material to
another

A

Refractive Index

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9
Q

Regarded as the Father of
Microscopy

A

Anton Van Leeuwenhoek (1632-1723)

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10
Q

One lens; Leeuwenhoek

A

Simple Microscope

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11
Q

First to observe Bacteria and
Protozoa using his microscope

A

Anton Van Leeuwenhoek (1632-1723)

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12
Q

Magnifies images from 100x to
300x

A

Simple microscope

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13
Q

Series of lenses

A

Compound Microscope

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14
Q

Uses visible light to observe specimen

A

Light Microscopy

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15
Q

Specimen appears against a bright
background

A

Brightfield Microscopy

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16
Q

Use: various stained specimens

A

Brightfield Microscopy

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17
Q

Special condenser with opaque disk.
Light enters the objective lens

A

Darkfield Microscopy

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18
Q

Use: for microorganisms which cannot
be stained

A

Darkfield Microscopy

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19
Q

Uses condenser with annular
diaphragm which allows direct light to
pass through the condenser

A

Phase Contrast

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20
Q

Use: detailed examination of internal
structures; does not require staining

A

Phase Contrast

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21
Q

Uses a UV or near-UV source of
illumination

A

Fluorescence

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22
Q

Use: observation of natural
fluorescence and
immunofluorescence

A

Fluorescence

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23
Q

Uses different refractive indexes to
produce image

A

Differential Interference

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24
Q

Use: provides 3D images of samples

A

Differential Interference

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25
Light source and condenser is on top, above the stage
Inverted
26
Use: observation of living cells under more natural conditions
Inverted
27
Beam of electrons instead of light
Electron Microscopy
28
Greater resolving power
Electron Microscopy
29
Electrons are reflected from the specimen; magnifies 1,000 to 10,000X
Scanning Electron Microscope (SEM)
30
Use: study of surface features of cells and viruses
Scanning Electron Microscope (SEM)
31
the Scanning Electron Microscope magnifies..
1000 to 10,000x
32
Electrons pass through the specimen; magnifies 10,000 to 100,000X
Transmission Electron Microscope (TEM)
33
Use: examination of viruses or internal ultrastructure in thin sections of cells
Transmission Electron Microscope (TEM)
34
The Transmission Electron Microscope (TEM) magnifies...
10,000 to 100,000x
35
Uses a thin metal probe that scans a specimen
Scanning Tunneling Microscope (STM)
35
Measures the deflection of a laser beam aimed at the tip of a probe that travels across the surface of the specimen
Atomic Force Microscopy
36
Use: reveals bumps and depressions of atoms on surface of specimen
Scanning Tunneling Microscope (STM)
37
Use: visualization of individual molecules and atoms
Atomic Force Microscopy
38
Used to contain cultures of microorganisms.
Screw-capped Tubes
39
Used to protect substances from contamination.
Screw-capped Tubes
40
Used to make broth, agar deep and agar slant.
Screw-capped Tubes
41
Detects production of gas by microorganisms.
Durham Fermentation Tube
42
Simpler than Durham tube, same use.
Smith Fermentation Tube
43
Used to make agar plates for microbiology study.
Petri Dish
44
Used to measure liquids to a precise volume.
Volumetric Flask
45
May also be used for storing liquids
Volumetric Flask
46
Has a single graduation
Volumetric Pipette
47
Delivers one specific volume accurately.
Volumetric Pipette
48
AKA blow-out pipettes
Serologic Pipette
49
Calibrated all the way to the tip so the last drop of the solution will have to be blown out of the pipette.
Serologic Pipette
50
Has no 0 mL mark
Serologic Pipette
51
Easier to deliver liquids with smaller volumes with precision and accuracy.
Pipettor
52
Tips are disposable
Pipettor
53
Used to observe specimens for a long time because they do not dry up very quickly.
Hanging Drop Slide
54
motility of an organism can be observed.
Hanging Drop Slide
55
AKA smear loop, inoculation wand, microstreaker
Inoculating Loop
56
used to retrieve and transfer an inoculum from a culture of microorganisms
Inoculating Loop
57
mainly used to pick a single colony (pure) and transfer in an agar slant (stab and streak method)
Inoculating Needle
58
Used to contain test tubes
Racks
59
Uses dry heat to sterilize heat-stable materials
Hot Air Sterilizer
60
For sterilization of media and other materials affected by moisture
Hot Air Sterilizer
61
Uses moist heat or steam under pressure
Autoclave
62
For sterilization of media and other materials affected by moisture
Autoclave
63
Not sterile
Drying Oven
64
CANNOT be used to sterilize culture media
Drying Oven
65
For drying specimens and lab apparatus or glassware
Drying Oven
66
used for growing bacterial cultures or providing suitable conditions for a chemical or biological reaction.
Incubator
67
It is used in the microbiological lab for incubations or enabling chemical reactions to occur at higher temperatures
Water Bath
68
Storage for culture media
Refrigerator
69
Preserves the morphological characteristics of microorganisms while arresting their growth
Refrigerator

micro lab (5 decks)

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