Midterm Topics: Chipchase and Birnstiel

Question 1

What is the question Chipchase and Birnsteil are answering?

Answer 1

Trying to answer what RNA interacts with Amino Acids

Question 2

What is a dual-y axis plot? How to read it?

Answer 2

Plot with 2 y-axis; read by looking at the key to know which side to use for which data points

Question 3

What RNA molecules are represented on the plot? Where are they shown?

Answer 3

tRNA: 16-18~ small but lots of it

mRNA: 4~ little but of big RNA

rRNA: 7,12~ LOTS OF IT IN MEDIUM SIZE

Question 4

What experimental techniques were used?

Answer 4

• Dumped RNA and 1st column which catches biggest RNA fragments; and then kept pouring till the smallest RNA was caught in the last column
• The beakers that were poured had the radioactive amino acid (leu) and that only got caught with tRNA because it only interacts with that RNA

Question 5

Major Conclusions?

Answer 5

We know tRNA binds to amino acids