Midterm Study Guide Pt. 1

Question 1

What are the basic parts of a compund light microscope?

Answer 1

arm
base
condensener  lens
coarse focus adjustment knob 
eyepiece (ocular lenses)
fine focus adjustment knob 
iris diaphragm/lever 
objective lenses 
mechanical stage 
stage clips 
fine focus adjustment knob 
rotating nosepiece 
mechanical stage adjustment 
rheostat

Question 2

What is the use of a compound light microscope?

Answer 2

Compound light microscopes use two sets of lenses - an objective lens and an eyepiece - to produce images.

Question 3

Compound light microscopes use two sets of lenses - an ________ ____ and an ________ - to produce images.

Answer 3

objective lens

eyepiece

Question 4

How do you figure out the total magnification of a compound light microscope?

Answer 4

total magnification= ocular magnification X objective magnification

Question 5

What is the total magnification of the objective magnification, 4X?

Answer 5

10X x 4X= 40X

Question 6

What are the steps to making a wet mount?

Answer 6

1. add a drop of water to a slide and place the specimen in the water
2. place the edge of the coverslip on the slide so it touches the edge of the water
3. slowly lower the coverslip to prevent formation and trapping of air bubbles
4. remove excess water from slide using lens paper

Question 7

What are the domains?

Answer 7

1. Domain Bacteria
2. Domain Archaea
3. Domain Eukarya

Question 8

What are the kingdoms of Eukarya?

Answer 8

1. protists
2. fungi
3. plants
4. animals

Question 9

What is an example of the protists kingdom?

Answer 9

• protozoans
• algae
• diatoms

Question 10

What is an example of the fungi kingdom?

Answer 10

• molds

- mushrooms

Question 11

What is an example of the plants kingdom?

Answer 11

• mosses

- ferns

Question 12

What is an example of the animal kingdom?

Answer 12

• vertebrates

- invertebrates

Question 13

What is a hypothesis?

Answer 13

an answer to scietific question

Question 14

What are the different types of variables?

Answer 14

Independent
Dependent
Cofounding

Question 15

Which type of variable is this: one factor given to one group but not the other

Answer 15

Independent Variable

Question 16

Which type of variable is this: additional variable that has not been controlled for, and may have affected the outcome

Answer 16

Confounding Variable

Question 17

What are the two groups in a controlled experiment?

Answer 17

(1) control group

(2) experimental group

Question 18

Which group in a controlled experiment receives the placebo?

Answer 18

control group

Question 19

What are the steps of the scientific method?

Answer 19

1. Observe
2. Hypothesize
3. Test
4. Interpret

Question 20

Which type of test is used to detect lipids?

Answer 20

Sudan Test

Question 21

Which type of test is used to detect proteins?

Answer 21

Biuret Test

Question 22

Which type of test is used to detect carbohydrates (or simple sugars)?

Answer 22

Benedict’s Test

Question 23

______ are insoluable in water.

Answer 23

Lipids

Question 24

Which test do you gently shake the solution to find the results?

Answer 24

Biuret Test

Question 25

How many drops of Sudan III do you add to each?

Answer 25

1-2 drops

Question 26

How many drops of Biuret reagent do you add to each tube?

Answer 26

3 drops

Question 27

Which test do you heat up to find the solution?

Answer 27

Benedict’s Test

Question 28

How many drops of Benedict’s reagant do you add to each tube?

Answer 28

20 drops

Question 29

How lond do you heat up to find the solution(s) in the Benedict’s Test?

Answer 29

5-10 minutes