Midterm Study Guide Pt. 1 Flashcards
What are the basic parts of a compund light microscope?
arm base condensener lens coarse focus adjustment knob eyepiece (ocular lenses) fine focus adjustment knob iris diaphragm/lever objective lenses mechanical stage stage clips fine focus adjustment knob rotating nosepiece mechanical stage adjustment rheostat
What is the use of a compound light microscope?
Compound light microscopes use two sets of lenses - an objective lens and an eyepiece - to produce images.
Compound light microscopes use two sets of lenses - an ________ ____ and an ________ - to produce images.
objective lens
eyepiece
How do you figure out the total magnification of a compound light microscope?
total magnification= ocular magnification X objective magnification
What is the total magnification of the objective magnification, 4X?
10X x 4X= 40X
What are the steps to making a wet mount?
- add a drop of water to a slide and place the specimen in the water
- place the edge of the coverslip on the slide so it touches the edge of the water
- slowly lower the coverslip to prevent formation and trapping of air bubbles
- remove excess water from slide using lens paper
What are the domains?
- Domain Bacteria
- Domain Archaea
- Domain Eukarya
What are the kingdoms of Eukarya?
- protists
- fungi
- plants
- animals
What is an example of the protists kingdom?
- protozoans
- algae
- diatoms
What is an example of the fungi kingdom?
- molds
- mushrooms
What is an example of the plants kingdom?
- mosses
- ferns
What is an example of the animal kingdom?
- vertebrates
- invertebrates
What is a hypothesis?
an answer to scietific question
What are the different types of variables?
Independent
Dependent
Cofounding
Which type of variable is this: one factor given to one group but not the other
Independent Variable
Which type of variable is this: additional variable that has not been controlled for, and may have affected the outcome
Confounding Variable
What are the two groups in a controlled experiment?
(1) control group
(2) experimental group
Which group in a controlled experiment receives the placebo?
control group
What are the steps of the scientific method?
- Observe
- Hypothesize
- Test
- Interpret
Which type of test is used to detect lipids?
Sudan Test
Which type of test is used to detect proteins?
Biuret Test
Which type of test is used to detect carbohydrates (or simple sugars)?
Benedict’s Test
______ are insoluable in water.
Lipids
Which test do you gently shake the solution to find the results?
Biuret Test
How many drops of Sudan III do you add to each?
1-2 drops
How many drops of Biuret reagent do you add to each tube?
3 drops
Which test do you heat up to find the solution?
Benedict’s Test
How many drops of Benedict’s reagant do you add to each tube?
20 drops
How lond do you heat up to find the solution(s) in the Benedict’s Test?
5-10 minutes
