Midterm: Isolation Technique Flashcards

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1
Q

What is the purpose of an isolation procedure?

A

To reduce the concentration of microbes by mechanically separating them using a semisolid agar

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2
Q

How many loop-fulls of bacteria are transferred r the Petri dish?

A

1

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3
Q

What is done to the inoculation loop after the initial load of bacteria is applied

A

It is sterilized

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4
Q

What is a CFU?

A

Colony forming unit

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5
Q

Why do microbiologists want samples form isolated colonies, rather than undifferentiated masses?

A

Mixed or contaminated cultures will give inaccurate as/or inconsistent results and cannot be reliably tested.

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6
Q

Why are isolation techniques needed when studying microbial specimens taken from patients in a medical setting or from environmental samples?

A

In a clinical setting there are lots of microbes and isolating the microbes allows for one to differentiate

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7
Q

How can isolation techniques be used to salvage a contaminated culture?

A

You can take the desired culture and isolate it and then isolate it again until it is no longer contaminated

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8
Q

What problems might arise when attempting to salvage a contaminated culture?

A

If a contaminated culture is left too long the contaminated may kill off the original species

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9
Q

What are the two common isolation techniques used in the microbiology lab?

A

The spread plate technique and the streak plate technique

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10
Q

Which isolation technique often involves diluted cultures?

A

The spread plate technique

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11
Q

Which isolation technique does not involve diluted cultures?

A

The streak plate technique

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