Midterm: Aseptic Technique

Question 1

What is aseptic technique?

Answer 1

Methods of handling microbial cultures, patient specimens, and other sources of microbes in a way that prevents infection of the handler, and others who may be exposed.

Question 2

Why is aseptic technique important in micro lab?

Answer 2

It prevents infection of the handler, others who may be exposed, and it avoided contamination of the cultures and materials beings used.

Question 3

What is the relative concentration of microbes in test tube cultures?

Answer 3

They have a high concentration

Question 4

Is the cap water tight?

Answer 4

No

Question 5

How do you aseptically transfer microbes to and from a test tube culture?

Answer 5

Sterilize the inoculation tool before transferring (allow for time to cool), place tool in microbes and transfer to another test tube, etc. (You know the rest of the process)

Question 6

What are the inoculation tools used in the lab?

Answer 6

Inoculation loop and inoculation needle

Question 7

Why is an inoculation loop used over an inoculation needle?

Answer 7

An inoculation loop picks up more microbes

Question 8

How would you know if the inoculation tool was sterile and ready for aseptic transfer?

Answer 8

The loop has to be passed through a Bunsen burner until red hot, then it has to cool for 10-15 seconds.