Midterm III - Helminthology 1 [INCOMPLETE] Flashcards

Trematoda: Egg

Trematoda: Miracidium inside the egg

Trematoda: Miracidium (released from egg)

Trematoda: Miracidia

Trematoda: Sporocyst

Trematoda: Sporocyst containing rediae

Trematoda: Sporocyst containing rediae

Trematoda: Redia

Trematoda: Cercaria

Trematoda: Cercaria

Trematoda: Full cercaria
Typical form of the blood flukes
[White spheres]

Trematoda: Metacercariae

Trematoda: Metacercaria

Trematoda: Metacercariae

Trematoda: Metacercaria
Notice the sucker in the middle

Trematoda: Migrant form of a young fluke

Fasciola hepatica (Common liver fluke)
Acute fasciolosis: Haemorrhages on serosa

Fasciola hepatica (Common liver fluke)
Haemorrhagic tracks in a sheep’s liver

Fasciola hepatica (Common liver fluke)
Haemorrhagic tracks in a sheep’s liver

Fasciola hepatica (Common liver fluke)
Chronic fasciolosis → Flukes are found in the bile duct

Fasciola hepatica (Common liver fluke)
Identifying features:
- Dorsoventrally flattened/Leaf-like
- Grey-brown
- Conical head shape (with shoulders)

Fasciola hepatica (Common liver fluke)
Identifying features:
- Dorsoventrally flattened/Leaf-like
- Grey-brown
- Conical head shape (with shoulders)
[See the yellow eggs]

Fasciola hepatica (Common liver fluke)
Identifying features:
- Dorsoventrally flattened/Leaf-like
- Grey-brown
- Conical head shape (with shoulders)
[Notice the yellow eggs]

Fasciola hepatica (Common liver fluke)
Identifying features:
- Parenchyma with dark intestinal cross-sections

Fasciola hepatica (Common liver fluke)
Identifying features:
- Red spikes on the surface = Trematode
- Oral sucker visible in the centre
*MIDTERM*

*Fasciola hepatica (Common liver fluke)*
Identifying features:
- Digested blood in the intestine = Liver fluke

Fasciola hepatica (Common liver fluke)
Identifying features:
- Cross-section showing intestine with digested blood

Fasciola hepatica (Common liver fluke) [Liver necropsy]
Identifying features:
- Chronic stage: Enlarged & fibrotic lesions

Fasciola hepatica (Common liver fluke)
Identifying features:
- Enlarged gall bladder & bile duct

Fasciola hepatica (Common liver fluke)
Identifying features:
- Enlarged bile duct wall

Chronic fasciolosis clinical sign:
- Submandibular oedema

Preserved eggs of liver fluke
- Detected using sedimentation

Radix labiata
Occasional host of Fasciola

Galba truncatula
Most common host for Fasciola & Calicophoron spp.

Galba truncatula
Infected with Fasciola larvae

Fascioloides magna (Great American liver fluke)
Identifying features:
- Dark areas are intestines with digested blood

Fascioloides magna (Great American liver fluke)
Caverns in the liver of a Red Deer

Fascioloides magna (Great American liver fluke)
Mass of eggs in the liver

Fascioloides magna (Great American liver fluke)
Mass of eggs in the liver

Rumen fluke
Either Paramphistomum or Calicophoron spp.

Rumen fluke
Either Paramphistomum or Calicophoron spp.

Rumen fluke
Either Paramphistomum or Calicophoron spp.
- 1 large ventral sucker; 1 smaller oral sucker

Rumen fluke
Either Paramphistomum or Calicophoron spp.
Identifying features:
- 1 large ventral sucker
- 1 smaller oral sucker
- Genital opening in the centre

Rumen fluke [Oral end]
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Ventral end]
Either Paramphistomum or Calicophoron spp.
Sucker is visible

Rumen fluke
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Ventral end] Cross section
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Oral end]
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Ventral end]
Either Paramphistomum or Calicophoron spp.

Planorbid snail
Intermediate host for Paramhistomum spp.

Effect of young rumen flukes moving in the s. intestine mucosa

Developing rumen flukes on the abomasal wall

Rumen fluke [Ventral end]
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Among ruminal papillae]
Either Paramphistomum or Calicophoron spp.

Rumen fluke [Among ruminal papillae]
Either Paramphistomum or Calicophoron spp.

Rumen fluke: Non-embryonated egg
Either Paramphistomum or Calicophoron spp.

Dicrocoelium dendriticum (Lancet fluke)

Dicrocoelium dendriticum (Lancet fluke)

Dicrocoelium dendriticum (Lancet fluke)

Dicrocoelium dendriticum (Lancet fluke)

Uterus w/ eggs

Vitellaria

Testes

Oral sucker

Ventral sucker

Dicrocoelium dendriticum (Lancet fluke): Egg
Hard & thick shell - Detect with floatation

Dicrocoelium dendriticum (Lancet fluke): Eggs

Landsnail: Intermediate host of:
Dicrocoelium dendriticum (Lancet fluke)

Dicrocoelium dendriticum (Lancet fluke): Cercaria
Found in slime balls on grass

Dicrocoelium dendriticum (Lancet fluke): Metacercaria

Dicrocoelium dendriticum (Lancet fluke)
Chronic dicrocoeliasis: Thickened bile ducts

Dicrocoelium dendriticum (Lancet fluke)
Chronic dicrocoeliasis: Thickened bile ducts

Dicrocoelium dendriticum (Lancet fluke)
This can be identified by:
- Presence of eggs

Dicrocoelium dendriticum (Lancet fluke)
This can be identified by:
- Presence of eggs

Dicrocoelium dendriticum (Lancet fluke)
This can be identified by:
- Presence of eggs
How to distinguish between Liver fluke and Lancet fluke?
- Liver fluke: Visible digested blood in the intestine
- Lancet fluke: Visible eggs present

Fasciola hepatica (Common liver fluke) [Liver necropsy]
Chronic fasciolosis: Adult flukes in the bile duct

Moniezia benedeni
Identifying features:
- Each proglottis has 2 sets of genitals which open on both sides

Anoplocephala perfoliata
Identifying features:
- Each proglottis has 1 set of genitals which all open on the same side
- Testicles are scattered as pale spots on the opposite side

Moniezia benedeni: Egg
Identifying features:
- Quadrangular egg shape
- Oncosphaera is eccentric

Moniezia expansa: Egg
Identifying features:
- Triangular egg shape
- Oncosphaera is central

Anoplocephala perfoliata: Egg
Identifying features:
- Semicircular shape
- Contains 6 hooked oncosphera inside

Dipylidium caninum (Flea/cucumber tapeworm): Mature segment
Identifying features:
- Genital openings on both sides (impressions on the surface)
- 2 x Ovaries in the centre
- Testicles fill the central part of the proglottis

Dipylidium spp.: Cocoon
Identifying features:
- Oncospheres fill the centre

Diphyllobothrium latum (Broad/fish tapeworm): Scolex
Identifying features:
- Longitudinal, oval slit (Bothridium)

Diphyllobothrium latum (Broad/fish tapeworm): Proglottids
Identifying features:
- 1 set of genitalia
- Genital openings in the midline

Diphyllobothrium spp: Eggs
Identifying features:
- Resemble the features of Trematode eggs
- No oncosphera

Taenia spp.: Rostellum
Identifying features:
- Strong hooks on the rostellum [Ø T. saginata]
- Impressions of 2 x suckers (acetabuli)

Taenia spp.: Proglottids
Identifying features:
- Genital organs open on each side indiscriminately
- Proglottis are stretched to become longitudinally narrow

Taenia spp.: Mature segment
Identifying features:
- Mass of scattered testicles
- 2 longitudinal excretory canals
- 2 genital ducts parallel to the genital opening

Taenia spp.: Gravid segment
Identifying features:
- Branches of uterus are filled with white eggs

Taenia spp.: Egg
Identifying features:
- Thick refractive eggshell
- Six-hooked oncosphere (not all hooks are visible)
- Colour: White-brown

- Taenia spp.: Cysticercus* larva
- Liver tissue*
You can tell it isn’t Echinococcus because there is no surrounding CT

Taenia spp.: Cysticerci
Cut surface of skeletal muscle

Taenia multiceps: Coenurus larva
White solices can be seen on the opened wall

Echinococcus granulosus: Adult
Identifying features:
- Scolex with a rostellum & suckers
- Short neck
- Immature segment & mature segment
- Gravid segment
- Centrally-aggregated eggs

Echinococcus hyatidosus
Identifying features:
- Spherical protoscolices attached to the wall of inner broad capsules

- Echinococcus granulosus*: Protoscolex
- No. hooks on protoscolex = no. hooks of the adult’s scolex

Echinococcus multilocularis: Cavernous structure
Sterile larva not containing any protosolices
Difference between a Cysticercus pisiformis & an Eccinococcus interlocularis necropsy specimin.
- Cysticercus pisiformis: Lesions appear “on” the liver
- Eccinococcus interlocularis: Lesions appear inside the liver

Dipylidium caninum

Dipylidium caninum: Dried segments

Anoplocephala perfoliata

Moniezia expansa

Mesocestoides spp.

Anoplocephala perfoliata: Worm

Moniezia expansa

Dipylidium caninum

Anocephala perfoliata

Cysticercus bovis

Cysticercus cellulosae

- Cysticercus pisiformis*
- Note the lesions appear “*on” the liver

(Taenia) Cysticercus pisiformis: Rostellum

Mesocestoides lineatus

Cysticercus tenuicollis: On a liver

Taenia pisiformis: Gravid segment

Cysticercosis in the rabbit’s liver

Taenia saginata: Egg

Cysticercus: Heart muscle

Taenia pisiformis**: Gravid proglottids

Taenia pisiformis?: Haemorrhagic tracks in the liver

Mesocestoides lineatus: Segment

Mature cysticerci on the peritoneal surface

Cysticercus?: In the heart muscle

Cysticercus cervi

Cysticerci pisiformis: Rabbit liver

Echinococcus multilocularis larva: Echinococcus alveolaris

Taenia-type eggs

Echinococcus multilocularis?: Hyatid cyst
with protoscolices inside

Echinococcus granulosus: Protoscolex

Echinococcosis interlocularis
Note the cysts are in the liver

Echinococcus hydatidosus
Liver sample

(Taenia) Cysticercus crassiceps

Echinococcus hyatidosus: Larvae

Cysticercus longicollis: Larva

Echinococcus granulosus: Cyst

Cysticercus tenuicollis: Larvae

(Taenia) Coenurus serialis: Larva
Muscle of Hare

Echinococcus: Cysts

Taenia serialis

Taenia solium: Cisticerci
Heart sample

Taenia spp.: Cysticercus

Taenia serialis

Echinococcus: Hydatid cyst (liver)

Taenia multiceps

Taenia saginata: Cysticercus bovis

Taenia hydatigena

Taenia multiceps: Coenurus larvae

Taenia hydatigena: Cysticerci

Taenia saginata: Worm & Larva

Taenia spp.

Taenia pisiformis

Taenia pisiformis

Taenia pisiformis: Gravid segment

Taenia pisiformis: Eggs

Taenia saginata: Eggs

Cysticercus tenuicollis
(Taenia hydatigena larval form)

Strobilocercus fasciolaris
(Taenis taeniaeformis larval form)

Cysticercus bovis

Cysticercus larvae in the brain

- Cysticercus longicollis*
- (Taenia crassiceps larval form)*

Coenurus cerebralis
(Taenia multiceps larval form)

Coenurus serialis
(Taenia serialis larval form)
Found in CT & muscles

Echinococcus granulosus

Echinococcus granulosus

Cysticercus pisiformis
(Taenia pisiformis larval form)

- Cysticercus cellulosae*
- (Taenia solium larval form)*

Taenia pisiformis

Taenia serialis

Cysticercus tenuicollis
(Taenia hydatigena larval form)