MIDTERM: APTT (lab)

Question 1

Detects coagulation factor deficiency in Intrinsic and Common pathway

Answer 1

Activated Partial Thromboplastin Time (APTT)

Question 2

Normal value for APTT

Answer 2

25-35 seconds (lab manual) or 35-45 seconds (PPT ni sire)

Question 3

It is the time required to generate thrombin and fibrin polymers in intrinsic pathway

Answer 3

Activated Partial Thromboplastin Time (APTT)

Question 4

Specimen used for APTT

Answer 4

Citrated blood

Question 5

Reagent used for APTT

Answer 5

Thromboplastin, Activator (Kaolin, Celite, Ellagic acid), Calcium chloride, Phospholipids

Question 6

Used for detecting the presence of circulating anticoagulants (inhibitors) and for monitoring heparin therapy

Answer 6

Activated Partial Thromboplastin Time (APTT)

Question 7

What are the factors present in the intrinsic coagulation mechanism that are not measured in APTT?

Answer 7

Platelets and Factor XIII

Question 8

Is Factor VII measured in APTT?

Answer 8

No. Because it in the extrinsic system

Question 9

This is bound by the sodium citrate anticoagulant to prevent coagulation

Answer 9

Calcium in the whole blood

Question 10

The plasma after centrifugation contains all intrinsic coagulation factors except?

Answer 10

Platelets and calcium

Question 11

In APPT, these are added to the plasma

Answer 11

Calcium and a phospholipids substitute

Question 12

These are much more sensitive to coagulation factor deficiencies than the whole blood clotting time

Answer 12

PT and APTT

Question 13

Abnormally prolonged PT and APTT are cause by?

Answer 13

• Deficiency in one of the coagulation factors involved in the intrinsic and common pathway
• Inhibitor of coagulation may be present

Question 14

An abnormally shortened APTT may be caused by?

Answer 14

• Partial clotting of the blood as a result of a traumatic venipuncture
• High levels of Factor 3
• Disseminated intravascular coagulation (DIC)
• Presence of platelets in plasma

Question 15

Measures the availability of functional fibrinogen

Answer 15

Thrombin Time

Question 16

Determine the rate of thrombin-induced cleavage of fibrinogen to fibrin monomers

Answer 16

Thrombin Time

Question 17

Reagent used in Thrombin Time

Answer 17

Thrombin reagent and Calcium chloride

Question 18

In Thrombin Time test, how many mL of plasma do you add in a plastic tube or siliconized glassware?

Answer 18

0.2 mL plasma

Question 19

Normal value in Thrombin Time

Answer 19

15-18 seconds

Question 20

Thrombin time is prolonged in:

Answer 20

• Fibrinogen deficiency
• Presence of Fibrin degradation products
• Presence of Thrombolytic agent
• Presence of Heparin
• Presence of Streptokinase

Question 21

Ducker’s Test (5M Urea Solubility Test) is used for?

Answer 21

Factor XIII deficiency

Question 22

Reagent used in Ducker’s Test (5M Urea Solubility Test)

Answer 22

5M Urea (or 1% monochloroacetic acid, 2% acetic acid)

Question 23

In Ducker’s Test (5M Urea Solubility Test), you add ___ mL of Calcium Chloride to ____ mL PPP.

Answer 23

0.2 mL on both

Question 24

In Ducker’s Test (5M Urea Solubility Test), You incubate the tube at?

Answer 24

37C for 30 minutes

Question 25

In Ducker’s Test (5M Urea Solubility Test), you observe for the dissolving clot in?

Answer 25

1, 2, 3, and 24 hours

Question 26

What are the normal and abnormal results in Ducker’s Test?

Answer 26

Normal: Clot is insoluble to Urea
Abnormal: Clot is soluble to Urea