Lec: Hema 2 Intro (katong docx) Flashcards
Method for controlling infection in which all blood and certain body fluids are treated as if infected with hepatitis B, human immunodeficiency virus (HIV) or other disease producing blood-borne pathogens.
Universal precaution
TRUE OR FALSE:
Any skin surfaces that become contaminated with blood or body fluids should be washed immediately. Hands are not necessarily washed upon removal of gloves.
FALSE. Hands must be washed upon removal of gloves
TRUE OR FALSE:
During phlebotomy, it is okay to recap the used needles
FALSE
All pipetting must be carried out using?
mechanical pipet devices
Minimum requirement of decontaminating all the laboratory work benches with appropriate germicide
At least once per shift. Ideal is every after work has been completed
Where do you place materials coming in contact with blood or body fluids
Biohazardous waste containers
The Medical Technologist most often comes in contact with a patient during?
Blood collection
What are the qualities of a good phlebotomist?
Skill, patience, understanding (っ＾▿＾)💨
Betadine when used to clean the phlebotomy area will cause some erroneous test results such as?
falsely elevated potassium, uric acid, and phosphorus results
Gauze and alcohol prep swabs should be disposed in?
biohazardous wasted containers
The puncture site should be cleaned by rubbing vigorously with a pad thoroughly
moistened with?
70% isopropanol (v/v)
This should not be routinely used to clean phlebotomy area because contamination
with this substance will cause some erroneous test results
Betadine
When vacutainer blood collection system is used, coagulation studies should be the ____ or ____ filled.
second and tube, respectively
If only coagulation tests
are ordered, a plain tube should be filled with _____ of blood first and then the
coagulation tube filled.
2-3 mL
In special coagulation
procedures, using a two-syringe technique, approximately how many mL of blood should be withdrawn into the first syringe?
2 mL of blood
(✿◠‿◠)
When do you remove the tourniquet when phlebotomizing the patient?
Release the tourniquet as soon as the blood enters the first tube (or the syringe, if used).
What is the anticoagulation of choice for coagulation studies?
0.109 M sodium citrate
(buffered or nonbuffered)
Ratio of blood to anticoagulant
9 parts
blood to 1 part sodium citrate
Blood specimens with a high hematocrit will contain?
less plasma
Its presence in the anticoagulant maintains a more stable pH
Buffer
Whenever a patient’s hematocrit is 55% or higher the amount of sodium citrate in the collection tube should be?
decreased
Formula used to
determine the proper amount of sodium citrate.
Amount of sodium citrate= 100 - Hematocrit/ 595 - Hematocrit multiplied with mL of whole blood used
This renders the specimen unacceptable for coagulation testing.
presence of a clot (no matter how small)
(^｡^)
The specimen should be centrifuged with _____ of obtaining the sample.
1 hour
All pipets, test tubes, and reaction cups that come in contact with the test plasma should have a?
“noncontact” surface
Why are plasma specimens in a stoppered container at all times? (except when testing)
To prevent loss of CO 2, and a resultant pH change of the plasma
For coagulation procedures, If there is to be a delay beyond 2 hours it is advisable to keep the specimen on?
Ice
Specimens that are maintained at room temperature are for:
Prothrombin time
Factor VII assay
Platelet function studies
This factor may become activated by the cold.
Factor VII
This is indicative of a traumatic venipuncture and should not be used for coagulation studies.
Hemolyzed plasma
Platelet poor plasma
plasma platelet count less than 15,000/µL
This process routinely produces acceptable platelet-poor plasma.
Centrifugation for 15 minutes at 2500 x g
Platelet-poor plasma may be stored at?
-40°C or lower for at least several weeks
When thawing frozen plasma it should be done rapidly in?
37°C incubator or water bath
TRUE OR FALSE:
It is acceptable to refreeze plasma
FALSE. Plasma cannot be refrozen.
＼（＾▽＾）／
Temperature for most coagulation studies
37°C.
This method requires gentle tilting of the tube back and forth at the rate of about once per second until a fibrin web is formed.
The tilt tube method
These procedures employ the use of automation.
Moving probe and Optical density
This is formed more slowly and may first appear as very fine fibrin threads.
Prolonged clotting times
These have been performed in duplicate and the two numbers averaged for a final result.
Almost all coagulation procedures
Drawbacks of double testing:
time-consuming and
expensive.
。ヾ(｡>ｖ<｡)ﾉﾞ。
