Lec: Megakaryopoiesis and Platelet Flashcards
IL-3, GM-CSF, and M-CSF are required for the conversion of?
Hematopoietic Stem Cell to Common Myeloid Progenitor
The factor that stimulates the conversion of megakaryocyte progenitor to erythrocytes
EPO (Erythropoietin)
Factors that stimulate the conversion to megakaryocyte erythroid progenitor
IL-3, SCF (stem cell factor), TPO (thrombopoietin)
IL-11 and TPO stimulates the conversion of Megakaryocyte Progenitor to?
Platelets
The largest hematopoietic stem cell in the bone marrow
Megakaryocyte/ Mature Megakaryocyte
This is unique only in the megakaryocytic series
Endoreduplication (endomitosis) (๑>◡<๑)
It is a process whereby the nuclear material reduplicates but the nucleus does not divide
Endoreduplication or Endomitosis
The three progenitor cells carry the marker?
CD34 (ノ^_^)ノ
What are the 3 Progenitor Cells?
- Burst forming unit (BFU-Meg)
- Colony forming unit (CFU-Meg)
- Light density- CFU (LD-CFU- Meg)
These carry the marker CD42
MK-I, MK-II, MK-III
Hormones/ cytokines responsible for the transformation of MK-I to MK-II (Differentiation to Megakaryocytes)
TPO, L-3, L-11
Hormones/ cytokines responsible for the transformation from granualr to mature ( Late maturation)
TPO, L-6_ L-11
Hormones/ cytokines responsible for thrombopoiesis
L-6, L-11
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IDENTIFY:
Cytoplasm is more abundant, less basophilic, and granules begin to form
Promegakaryocyte
IDENTIFY:
Cytoplasm contains coarse clumps of granules aggregating into little bundles, which bud off from the periphery to become platelets.
Mature megakaryocyte
IDENTIFY:
Its nucleus is round, oval and has multiple nucleoli
Megakaryoblast
IDENTIFY:
Multiple nucleoli are visible.
irregular in shape. may show lobulation.
Promegakaryocyte
☝( ◠‿◠ )☝
It is the first stage in the Megakaryopoiesis that does not contain nucleolus
Granular Megakaryocyte
IDENTIFY:
Nucleus: Multiple nuclei or may show multi-lobulation, Chromatin is coarser. No nucleoli are visible.
Granular Megakaryocyte
Occurs as a reserve in case of trauma in the body (ex. hemorrhagic or unexpected bleeding)
Splenic Platelet Pool
IDENTIFY:
Nucleus: Multilobulated. No nucleoli visible.
Mature megakaryocyte
IDENTIFY:
2-4 um in size, Cytoplasmic fragments of megakaryocyte and it has no nucleus
Platelets (Thrombocytes) ٩(●ᴗ●)۶
What is the function of platelets?
Primary Hemostasis and Maintaining Capillary Integrity
Platelet normal range:
150,000-450,000/ uL
Platelet normal function
> 100,000/uL
Hemorrhage happens if the platelet count is?
<10,000/uL
These are major glycoproteins responsible for Primary Hemostasis
GP Ib and GP IIb/ IIIa Complex
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This part of the platelet structure is composed of the membranes and is responsible for platelet Adhesion and Aggregation
Peripheral zone
This is composed of the Mitochondria, Alpha granules, dense bodies (delta granules)
Organelle Zone
Invaginations of the plasma membrane for release of granule content during platelet activation process.
Surface Connecting system (Open Canalicular system)
Composed of protein tubulin that maintains the
shape of platelets
Microtubules ┗|・o・|┛
Glycoprotein responsible for Platelet Adhesion
GP Ib
Glycoprotein responsible for Platelet
Aggregation
GP IIb/ IIIa Complex
A Cofactor in Fibrin Formation is released from?
Alpha Granules
Which Zone has the receptors necessary for platelet Aggregation?
Peripheral Zone
Granule formation occurs in?
Promegakaryocyte
୧〳 ＾ ౪ ＾ 〵୨
One of the sources of Calcium during Platelet aggregation can be found in?
Dense Tubular system
Von Willebrand Factor (vWF) participates in?
Platelet Adhesion
Responsible for Platelet shape:
Sol Gel Zone and Structural Zone
Provide negative charged phospholipid surface for Factor V and II activation
Platelets
Endoplasmic Reticulum that sequesters calcium for Platelet activation
Dense Tubular System
PF3 is found in which component of the Platelet?
Peripheral Zone
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