MIDTERM 2 Cytochrome C

Question 1

What does respiratory catabolism involve

Answer 1

They involve enzymes that rapidly break down (oxidize) compounds such as sugars via the EMP pathway and teh TCA cycle.

Question 2

What is oxidative phosphorlyation?

Answer 2

Electrons are passed from one cytochrome to another and with each step they transfer some of their energy to the phosphorylation of ADP to ATP

Question 3

What is the purpose of the terminal electron acceptor?

Answer 3

it removes the energy spent electrons, which are no longer of any use to the cell

Question 4

What is Needed for both catalase and cytochrome c test?

Answer 4

A fresh culture

Question 5

What will the presence of cytochrome C prove?

Answer 5

a microbe has the capacity for respiratory catbolism

Question 6

How can we identify for Cytochrome C oxidase test work?

Answer 6

1. add Tetramethyl-p-phenylenediamine ( colorless reagent in reduced state_
2. the reagent will be oxidize when the electrons are transferred to cytochrome C Changing its color to a dark purple compound known as wuster blue.

Question 7

Positive result for cytochrome C test?

Answer 7

Deep violet/purple color within 10 seconds