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Proc 180 > Mid 1 > Flashcards

Mid 1 Flashcards

1
Q

5 common glassware

A

High thermal resistant
High silica
High alkali resistant
Low actinic
standard flint

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2
Q

high thermal glass type

A

borosilicate

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3
Q

high thermal used for

A

beakers, flasks, pipettes

Resists heat changes

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4
Q

silica used for 3

A

optics, mirrors, cuvettes, thermometers

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5
Q

alkali glass, and used for

A

aluminosilicate
screen protectors- scratch resistants, alkaline resistant / corrosion

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6
Q

standard flint glass, and used for

A

soda lime
cheap: glass ware single use and dishes

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7
Q

5 plastic wares

A

polyolefins, polycarbonate, tygon (polyvinyl chloride), teflon, polystyrene

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8
Q

polyolefins 2 subs

A

polyethylene
polypropylene

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9
Q

polyolefins characteristics and used for

A

-unaffected by strong chemicals
-disposable: urine tubes, sample tubes, pipettes, bottles
-some autoclaves

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10
Q

polycarbonate characteristics and used for 2

A

-strong by susceptible to chemicals
-autoclavable
-centrifuge tubes and graduated cylinders
-not disposable

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11
Q

tygon 3 charac/uses

A

-non toxic
-flexible
-used in tubing
-clear
-handles most chemicals

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12
Q

teflon charac/purpose

A

-tough resin
-non-wettable/ is repelelnt
-stirring bars, stopcock, caps, tubing
-chemic innert

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13
Q

polystyrene charac/purpose

A

-chemic inert
-transparent
-not autoclavable
-microliter plates, petri dishes

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14
Q

most accurate flasks are

A

volumetric

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15
Q

graduated cylinders

A

less accurate but verstitile

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16
Q

beakers

A

not calibrated,
used as holding containers

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17
Q

Erlenmeyer flask

A

not calibrated
used for mixing to eliminate vapour

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18
Q

funnels

A

support filter

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19
Q

cuvettes

A

used in light measurement

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20
Q

fritted glass funnels

A

made from fine glass and different porosity used with filter paper, easy clean

-filters strong acids and bases

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21
Q

separating funnels

A

-separate immiscible liquids
-drop by drop filtering

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22
Q

test tubes can be made from

A

glass or plastic

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23
Q

centrifuge tubes

A

used to separate solids and liquids
plastic or though glass

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24
Q

3 types of cuvettes/ material

A

-disposable polystyrene
-quartz
-silica

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25
Q

storage bottles characteristics

A

cylindrical
narrow necks
ground glass stoppers or screw tops

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26
Q

burets used for

A

titration or standardization procedure
drop by drop

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27
Q

desiccators used for

A

drying out reagents and solids

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28
Q

2 types of desiccants and color

A

drierite
barium oxide

blue is dry pink is wet

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29
Q

non volumetric vs volumetric

A

not used for accuracy (just to aproximate) vs is

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30
Q

what class should all volumetric ware be in

A

class A analytic quality

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31
Q

To contain vs
to deliver

A

to contain holds some liquid at the end that must be blown out, to deliver if drained properly does not need to be blown out

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32
Q

temp calibration for ware

A

20 C

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33
Q

fourth part on the glass ware is

A

units, ml ususally

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34
Q

volumetric glassware 3 ex

A

volumetric flasks,
graduated cylinders
pipette

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35
Q

3 steps to clean glass

A

-group
-hot water
-brush

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36
Q

new glasses are what and must be what

A

-alkaline
-soaked in acid water
-and rinsed

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37
Q

contaminated glass clean with

A

-10-20% bleach

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38
Q

last rinse should always be with

A

-distilled water

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39
Q

cloudy, dirty, coagulated organic matter cleaned with

A

chromatic acid

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40
Q

protein cleaned with

A

ammonium hydroxide

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41
Q

grease cleaned with

A

sodium carbonate

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42
Q

heavy metals cleaned with

A

dilute nitric acid

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43
Q

capacity of pipetted

A

1 uL to 100 mL

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44
Q

semi-automated capacity

A

100 uL-1.0 mL

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45
Q

macro capacity

A

> 0.1 mL

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46
Q

micro capcacity

A

<0.1 mL (100 uL)

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47
Q

concave meniscus for … vs convex

A

clear
opaque

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48
Q

mohr vs sero vs volumt TC or TD

A

mohr TD
sero TC
volum TD

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49
Q

tolerance

A

what liquid is actually being delivers

%/100 x pipette mL = x minus pipette mL

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50
Q

tissue that wipes

A

Kimwipe

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51
Q

wipe semi auto only when and after always

A

working with blood
rinse out

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52
Q

6 thermal equipment
Hot and cold

A

refrigerators
freezers
water bath
heating block
air incubators
hot air oven

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53
Q

refrigerators
-temp
-used for 3

A
  • 4+- 2
    -short term samples
    -blood for transfusions
    -reagents
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54
Q

freezers temp and use for 4

A

-20 or lower
-long term plasma/serum
-reagents
-blood
-cultures

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55
Q

water baths temp and in water reagents and dis/adv

A

37-56
-fungicide: thymol/thimersol
-adv: quick heat and slow cooling
-dis: the water in bath takes time to warm up and and its big

56
Q

heating blocks temp and adv and dis

A

37-56
-adv: nothing gets wet
-dis : less efficient heat transfer

57
Q

air incubators temp , 2 types, usesful bc

A

37-56
-water jacket
-anhydric
-holds larger volume

58
Q

hot air over temp used for

A

160
drying glass ware

59
Q

microwaves

A

heat quickly and evenely

60
Q

continous temp logs for 4

A

Everything / thermometers in all
incubators, fridge, freezer

61
Q

centirfuges

A

seperate materials based on densitiy
rotation aroun central axis

62
Q

resisitance will what to speed

A

decrease it

63
Q

trunnion rings

A

hold buckets

64
Q

buckets

A

hold test tubes

65
Q

cushions

A

bottom oof bucket to prevnt breackage

66
Q

two broad categ of cenrifuges

A

fixed angle and horizontal head

67
Q

horizontal head 2 dis

A

greater resistance= heat, some remixing since we go from straight to side to straight

68
Q

fixed angle effcient bc

A

45 degreee
-less resistnace
-can operate at higher speed

69
Q

8 types of centrifugess

A

clinical
floor model
haematocrit
ultra
mega
serofuge
cell washer
refrigerated

70
Q

clinical
-character
-voulume

A

-small
-routine
-15-30 mL

71
Q

floor model

A

-large volumes
-many tubes
-high speed/ better

72
Q

haematocrit
for and force

A

-small tubes
-red cells bottom
-14,000

73
Q

ultracentirfuge
speed and for

A

-60,000
-special urine
-seperates densities

74
Q

megafuge
-use and spee

A

-large range of motors
-routine
-100-6000 rpm

75
Q

serofuge

A

-serology
-fixed angle
-fast to reach speed

76
Q

cell washer

A

-washes RDB with saline
-decant to supernantant

77
Q

refrigerated
temp
used

A

-15-25
-high speed but specimens not damaged from heat

78
Q

how should u increase speed

A

gradually

79
Q

clean centrifuge by

A

-bowl, head, accessories
-dry cloth
-mild detergent
-drying oven
-routine disinfection

80
Q

how long bf open cent whne break

A

30 min

81
Q

disinfect using what for cent

A

non-bleach

82
Q

mechanical balance

A

relies on removable weight
compensation load

83
Q

electronic balance

A

electric current
current is proportional to weight

84
Q

vernier scale is used for
capacity in g

A

weights on some mech balances
microscopes
0.01 g

85
Q

analytical balance
fine vs micro vs moderate vs rough

A

most accurate measurments
0.0001
0.000001g
0.005-0.1
1

86
Q

PH meters measure

A

H ion acitivty

87
Q

2 types of electrodes and diff and made of

A

reference -constant voltage does not respond - silver chloride
indicator - glass responds to H ions

88
Q

combination elctrode

A

both in one body

89
Q

2 sources of elctorode error

A

1- temp
2- reactive glass responds to more than H

90
Q

electrode care

A

soack in neutral budder
dont let glass dry out
clean and gentle

91
Q

LO1

A
92
Q

is 0 odd or even

A

even

93
Q

the round rule for 5 and 50

A

even always wins odd needs help

94
Q

when multiplying integenrs

A

add exponents

95
Q

when dividing integers

A

subtract exponents
what you have - what you want

96
Q

accuracy def

-certainty
-efficiency
-truthfulness

A

correctness/ clossness to true value

97
Q

precisions

A

reproducibility

98
Q

between 1.3 and 1.27489 which is more precise

A

larger number

99
Q

SI
length
mass
volume
time
amount
electric current
thermodynamic temp

A

m
g
L
s
mol
A
K

100
Q

in lab instead of K we use

A

C

101
Q

numeric date order

A

year-month-day

102
Q

time always has how many digits

A

4

103
Q

prefixes order 13

A

Tera
Giga
Mega
Kilo

hecto
deca
deci
centi

milli
micro
nano
pico
fecto

104
Q

hecto

A

2

105
Q

deci

A

-1

106
Q

deca

A

1

107
Q

centi

A

-2

108
Q

tera

A

12

109
Q

mega

A

6

110
Q

giga

A

9

111
Q

kilo

A

3

112
Q

milli

A

-3

113
Q

nano

A

-9

114
Q

micro

A

-6

115
Q

pico

A

-12

116
Q

fecto

A

-15

117
Q

the numerical value should be between what

A

0.1 and 1000

118
Q

should there be space btw unit and number

A

yes

119
Q

solution

A

solute dissolved in a liquid solvent

120
Q

aqueous vs non-aqueous

A

solvent is water vs
solvent alcohol/ organic liquid

121
Q

solute

A

substance dissolved in solvent to make solution

122
Q

solvent

A

the liquid

123
Q

dilute solutions

A

a small proportion of solute in solution
ex 0.85%

124
Q

concentrated solutions

A

large prop of solute

ex 20%

125
Q

saturated solutions

A

as much solute as solvent can dissolve
ex 40%

126
Q

supersaturate solutions

A

contains more solute than the solvent can keep dissolved

127
Q

diluent

A

liquid into another liquid, usually the smaller one

makes something fluid

128
Q

dilution

A

taking a concentrated solution and adding diluent to it to make it less concentration

129
Q

dilutions are statements of relative

A

concentrations not volume

130
Q

ratio and proportions vs dilutions

A

the ratio is a 1 to x
1:9
and proportion would be 2:18

dilution is a 1 in x
1:10

131
Q

concentration def

A

how much solute in a given volume

132
Q

%w/v vs %v/v

concentrations

A

g/100 mL
mL/ 100mL

133
Q

the weight of a substance is

A

the sum of the atomic weight of all atoms

134
Q

hygroscopic or deliquescent are when

how to dry

A

anhydrous substances absorb moisture from air and not 100% pure

-drying oven or desiccator

135
Q

hydrated chemicals are

A

substances crystalize in a way to unite with water and form hydrates

Proc 180 (4 decks)

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