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Proc 180 > lab safety > Flashcards

lab safety Flashcards

1
Q

most danger route and uderestimated of exposure

A

inhalation of aerosoles

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2
Q

how many risk groups are there

A

4

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3
Q

most common risk contamination level in reserach lebs

A

2

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4
Q

what are aersoles

A

suspensions of solid or liquid particles that can enter resp

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5
Q

5 factors that determine infection due to aerosoels

A

viability of biohazard
conctration of aeorosoles
size
presisitance
susceptibility of host

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6
Q

3 ways to decontaminate lab waste

A

autoclave
disinfect
incinerate

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7
Q

temp of autoclave

A

121

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8
Q

most common disinfect for non met

A

belach, sodium hypochlorite

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9
Q

equipment that is used when working with agents that make aerosols

A

biosafety cabinets

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10
Q

test microgorganizm in auticlaves

A

geobacillus stearothermophilus

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11
Q

hepa stands for

A

high efficiency particulate air

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12
Q

standarsd for hepa filters are based on

A

removal of particles of 0.3 um

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13
Q

hepa is made from

A

densely filter of glass and paper fibers on aluminum or wood base

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14
Q

safety cabinets are tested whe 3

A

annualy, initially, moves/repairs

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15
Q

class of biological safety cabinets is based on 3

A

protection
air circulation
closed system and hepa filter type

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16
Q

class 1, 2 a1, a2, b1 issues

A

recirculation of air , toxins / flamable inot cabinet

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17
Q

leacking spciments clean up 7 steps

A

inform others
PPE
place speciment onto tray and move to cabinet
clean contamianted surface
in cabinet transfers contents to clean container
if requisutions is contaminated move to plastic bag
flood new tray and disinfect/ biohaz

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18
Q

non fixed slides are dangerous ?

A

yes

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19
Q

sds stands for

A

safety data sheets

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20
Q

5 things mandatory on sds

A

identification
handling
first aid
toxicolofy
physical and chemical properties

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21
Q

flammable limits

A

the range at which fuel will burn if ignitions source is there

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22
Q

LC50

A

concentration of substance in air will kill 50% animals

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23
Q

LD50

A

quantity of a substance death to 50% animals

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24
Q

TWA

A

concentration in air that will not harm if exposed to for 8 hrs

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25
Q

STEL

A

15 min, for up to 4 times a day with no adverse affects

26
Q

ceiling limit

A

should never be exceeded, toxic

27
Q

flashpoint

A

concentration vaopur and soruce of ignition

28
Q

4 types of aeorosoles

A

mists
dusts
smoke
fumes

29
Q

human carcinogen in lab

A

benzene

30
Q

2 chmicals suspected of cancer

A

ethylene oxide
formaldehyde

31
Q

flammable

A

burns at room temp

32
Q

combustible

A

must be heated before burn

33
Q

oxidizing

A

material can provide oxygen to burn

34
Q

corrosive

A

damage body tisssue

35
Q

3 points to store oxidizers

A

away from flamables
closed lids
compatibility

36
Q

does corrosive material just dosent effect pyrex glass

A

yes

37
Q

are acids and basses corossive

A

yes

38
Q

are reactive chemicals just alwyas dangerous

A

yes

39
Q

danger chem in dry state

A

picric acid

40
Q

laser means

A

light amplification by stimulated emission of radiation

41
Q

is there no danger with laser

A

false there is

42
Q

class A fire extin

A

paper, tectiles, plastics

43
Q

class B fire

A

Oils

44
Q

class C fire

A

electiric

45
Q

class D fire

A

patassium metals

46
Q

cryogenics fluids and ex

A

gasses that have been liquefied at low temp
nitrogen

47
Q

3 equipment when working with liquid nitrogen

A

full face mask
imprevious clothing
water proof gloves

48
Q

egronomics keeps who in mind

A

worker

49
Q

egronomics does not supoert 1

A

bending head

50
Q

should wrists be raised up when typing

A

neur

51
Q

breaks every 3/4 hrs for 2-3 min ?

A

neur, every hr

52
Q

3 egroniics at computer

A

use wirist rest when not typing
kevel monitor
key board and mouse adjustable height

53
Q

how long bf opening centrifuge

A

30 min

54
Q

substance that cause birth defect

A

teratogenic

55
Q

causes embryo harm

A

embryogenics

56
Q

DNA harm

A

mutagenics

57
Q

harm to fetus

A

fetagenic

58
Q

w/ isolation patietns use

A

mask

59
Q

when handling materials use

A

gloves

60
Q

when workking with splatter use

A

apron

Proc 180 (4 decks)

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