microscopy RP

Question 1

aim

Answer 1

Use a light microscope to investigate a selection of plant and animal cells.

Question 2

method

Answer 2

• 1) use the pipette to put one drop of water on the microscope slide
• 2) separate one of the thin layers of the onion and make sure its flat on the slide
• 3) peel off layer of epidermal tissue and use forceps to put it on the drop of water
• 4) put 2 drops of iodine solution onto the onion tissue
• 5) place coverslip on the slide and put the slide on microscope stage
• 6) turn the nosepiece to the lowest power objective lens
• 7) look into the eyepiece and turn the fine
  adjustment knob until the image comes into focus.

Question 3

one safety precaution

Answer 3

Wear safety goggles when handling the iodine solution

Question 4

first 2 steps

Answer 4

• 1) use the pipette to put one drop of water on the microscope slide
• 2) separate one of the thin layers of the onion and make sure its flat on the slide

Question 5

step 3

Answer 5

• 3) peel off layer of epidermal tissue and use forceps to put it on the drop of water

Question 6

what do you do after you put epidermal tissue on the drop of water (step 4)

Answer 6

• 4) put 2 drops of iodine solution onto the onion tissue

Question 7

what comes before step 5

Answer 7

put the iodine solution on the onion tissue (step 4)

Question 8

what do you do after putting two drops of iodine solution on the onion tissue (step 5)

Answer 8

5) place coverslip on the slide and put the slide on microscope stage

Question 9

what do you do after putting the slide on the microscope stage

Answer 9

• 6) turn the nosepiece to the lowest power objective lens

Question 10

what do you do after turning the nosepiece to the lowest power objective lense

Answer 10

• 7) look into the eyepiece and turn the fine
  adjustment knob until the image comes into focus.