Microscopy (Photobiophysics II)

Question 1

Resolution definition

Answer 1

R = 0.61 λ / NA

Question 2

snells law of reflection Equation

Answer 2

n1 sin1 0 = n2 sin2 O (n1 = incident index) (n2 = refracted index) (01 = incident angle) (02 = refracted angle)

Question 3

Linsengleichungen

Answer 3

1/f = 1/g + 1/b f= Brennweite | g = Gegenstandsweite |
b = Bildweite + check diagram on Notes
F depends on wavelength

Question 4

Problem:chromatic and spherical aberration by lenses (image distortion due to optical system)

Answer 4

chromatic aberration Solution: lens doublet, compound lens designs

Question 5

Numerical Aperture (NA) and equation

Answer 5

measure of ability to collect light
NA=n⋅ sinμ
NA = λ/ 2d

Question 6

SLIDE 68 of VL 7 afters

Answer 6

Not learnt