Microscopy and Stains

Question 1

Magnification

Answer 1

the magnifying power of the lens

Question 2

Resolution

Answer 2

min distance you can have between two objects and still be able to tell that they are separate structures

Question 3

Light Microscopy

Answer 3

using light to observe tissues

Question 4

Electron Microscopy

Answer 4

using the excited electrons to see things (ten fold smaller)

Question 5

hematoxylin

Answer 5

stains basophilic blue (ribosomes, DNA, RNA)

Question 6

Masson

Answer 6

trichrome. Stains collagen blue green

Question 7

aldehyde fuchsin

Answer 7

elastic fibers are purple

Question 8

eosin

Answer 8

acidophilic red (mitochondria, cytosol, erythrocytes).

Question 9

Mallory

Answer 9

trichrome. collagen is bluish green

Question 10

Silver stains

Answer 10

reticular fibers are black/brown

Question 11

Orcein

Answer 11

elastic fibers are brown

Question 12

Periodic Acid-Schiff

Answer 12

carbohydrates are magenta

Question 13

Best’s Carmine

Answer 13

glycogen is crimson

Question 14

Osmium Tetroxide

Answer 14

unsaturated lipids are black

Question 15

Sudan Black B

Answer 15

lipids are gray/black

Question 16

Oil Red O

Answer 16

lipids are red

Question 17

Methyl Green-pyronine

Answer 17

DNA (green) and RNA (pink)