Microscopy and Magnification

Question 1

What is the magnification of a microscope ?

Answer 1

it is calculated by multiplying the eyepiece power by the objective power.
-if we have a ×10 eyepiece and we have selected a ×15 objective lens the magnification is ×150.
-this means the object has been magnified 150 times.

Question 2

Different conversions (smaller)

Answer 2

cm ×1000 mm ×1000 nanometers.

Question 3

Different conversions (larger)

Answer 3

nanometers ÷1000 mm ÷1000 cm ÷1000 m ÷1000 km ÷1000 t

Question 4

How do you calculate actual size ?

Answer 4

actual = image size/magnification.

Question 5

How do you calculate magnification ?

Answer 5

magnification = image size/actual size.

Question 6

How do you calculate image size ?

Answer 6

image size = actual size × magnification

Question 7

What is resolution ?

Answer 7

-and electron photomicrograph is clearer than a light micrograph.
-this is because it has greater resolution.

Question 8

Resolution…..

Answer 8

-the clearer the image the greater the resolution.

Question 9

What is the max resolution of a light micrograph ?

Answer 9

200nm

Question 10

Why can ribosomes not be seen ?

Answer 10

-because they can’t be seen because they’re (or under) 25nm.