Microscopy and Magnification Flashcards

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1
Q

What is the magnification of a microscope ?

A

it is calculated by multiplying the eyepiece power by the objective power.
-if we have a ×10 eyepiece and we have selected a ×15 objective lens the magnification is ×150.
-this means the object has been magnified 150 times.

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2
Q

Different conversions (smaller)

A

cm ×1000 mm ×1000 nanometers.

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3
Q

Different conversions (larger)

A

nanometers ÷1000 mm ÷1000 cm ÷1000 m ÷1000 km ÷1000 t

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4
Q

How do you calculate actual size ?

A

actual = image size/magnification.

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5
Q

How do you calculate magnification ?

A

magnification = image size/actual size.

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6
Q

How do you calculate image size ?

A

image size = actual size × magnification

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7
Q

What is resolution ?

A

-and electron photomicrograph is clearer than a light micrograph.
-this is because it has greater resolution.

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8
Q

Resolution…..

A

-the clearer the image the greater the resolution.

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9
Q

What is the max resolution of a light micrograph ?

A

200nm

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10
Q

Why can ribosomes not be seen ?

A

-because they can’t be seen because they’re (or under) 25nm.

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