Microscopy Activities Worksheet Flashcards

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1
Q

The arm is located where?

A

The area between the tube and the base.

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2
Q

How do you properly carry a microscope?

A

Grip the arm with one hand and place the other hand underneath the base

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3
Q

Where is the base?

A

The bottom support structure of the microscope.

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4
Q

What is the body? What does it include?

A

Main section of the microscope minus the base and illuminators. Includes the eyepiece and objective lenses but not focusing block.

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5
Q

What is the Coarse Focus?

A

The larger of the focusing knob, it adjusts the distance to the specimen in large steps.

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6
Q

Describe the diaphragm and what it does.

A

Five-hole disc housed under the stage to adjust the amount of light passing through the stage opening.

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7
Q

Describe the stage openings and their role.

A

Lets light pass through to properly illuminate the specimen, while increasing contrast and resolution.

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8
Q

The eyepiece is made up of what lenses and what magnification levels do they include?

A

Ocular lenses with magnifications of 5X, 10X ,15X, and 20X.

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9
Q

Which is the Fine Focus, what does it do, and when do you use it?

A

Smaller of the adjustment knobs that adjusts the distance from the specimen in small steps towards various parts of the a specimen.

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10
Q

What does the Nose Piece hold and what does it do?

A

Structure that holds the objective lenses. Can be rotated to switch the lense.

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11
Q

How can you identify the objective lens? Which is the scanning lens? What other lenses are there?

A

Closes lense to the object being viewed. With different lenses at different magnification: Scanning (4X), Low-power (10X), Mid-power (40X), and High-power lense (100X).

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12
Q

On/Off controls what?

A

Controls the power to the microscope.

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13
Q

What is the stage and what is its function?

A

Flat plate that holds the slides for observation.

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14
Q

What is the power of the typical eyepiece lens?

A

Typically 10X.

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15
Q

When would you use a 100X lens?

A

Within oil immersion.

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16
Q

What color is assigned to identify the resolution of each objective lens?

A

Red (4X), Yellow (10X), Blue (40X), White (100X).

17
Q

How do you calculate magnification of the image? An eyepiece of 10x and objective lens of 40x gives what total magnification?

A

Multiply the power of the eyepiece by the power of the objective lense. 10X * 40X would be 400X total magnification.

18
Q

What lens is typically used with oil? What does the oil do?

A

100X, The immersion oil increases their resolution.

19
Q

Describe the process of using oil. The oil serves as what?

A

A drop of oil is place on on the specimen slide. The oil now serves as a bridge between the lens and the glass palte contianing the specimen helping to focus the light and increase the resolution.

20
Q

When do you remove the dust cover?

A

Until the microscope is place and positioned on the table where it will be used.

21
Q

Can you wipe the lens with your finger? Why not?

A

No, oil from your hands could smudge the lens of even scratch it.

22
Q

What do you use to clean lens?

A

Lens tissues.

23
Q

What do you do to microscope when prepared to store.

A

Nosepiece should be in its lowest power setting, powest position to the satge. and the power cord neatly wrapped up, the dust cover should be replaced.

24
Q

How does a condenser lens help?

A

Helps increase illumination and resolution by focusing/condensing light onto the specimen.

25
Q

What is the cover slip and what does it do?

A

Thin square plastic or glass sued to cover the specimen on a slide.

26
Q

What is resolution?

A

A lens system’s ability to resolve find details of the observed object.

27
Q

What is the stage adjustment knob.

A

Mechanical stage allows for adjustment of the slide position forward, backward, and left or right.

28
Q

What does the X stand for in a lens with a 200X?

A

Represents the magnification factor.