Microscopy

Question 1

How does a Light Microscope work?

Answer 1

It uses light and lenses to form an image of a specimen and magnify it.

Question 2

What does a light microscope allow us to see?

Answer 2

Individual cells, large subcellular structures like Nuclei

Question 3

How does an Electron Microscope work?

Answer 3

They use Electrons instead of light to form an image.

Question 4

Why would somebody use an Electron microscope over a Light Microscope?

Answer 4

Electron microscopes have a higher magnification, higher resolution and they also let the user see smaller things in more detail

Question 5

Give an example of what an Electron Microscope would be used to observe?

Answer 5

Internal structures of Mitochondria and Chloroplasts, Ribosomes or plasmids.

Question 6

What is the formula for magnification?

Answer 6

Magnification = image size ÷ real size

Question 7

List the steps needed to look at a specimen under a Light Microscope

Answer 7

1. Put specimen on a Microscope slide
2. Add a drop of water to the middle of a clean slide
3. Place cells on to the water on slide
4. Add a drop of iodine solution
5. Place a cover slip on top

Question 8

What is a slide?

Answer 8

A strip of clear glass or plastic into which a specimen is mounted