Microscopy

Question 1

Magnification

Answer 1

Number of times larger an image appears compared with the size of the object

Question 2

Resolution

Answer 2

The degree of detail visible

Question 3

Purpose of staininh

Answer 3

Makes cells visible
Makes organelles visible
Enables different type of cells to be distinguished

Question 4

Light microscope pros

Answer 4

Cheap
Easy to use
Observe living specimen

Question 5

TEM pros

Answer 5

High mag
High resolution

Question 6

SEM pros

Answer 6

High mag
High res
3d image
Surface

Question 7

TEM cons

Answer 7

Dead
Expensive
Need training to use

Question 8

TEM cons

Answer 8

Dead
Expensive
Need training to use

Question 9

How sem works

Answer 9

Electron beam is directed onto sample

Question 10

How tem works

Answer 10

Electron beam passes through sample

Question 11

How can specimen be preserved

Answer 11

Dehydrated
Embedded in wax
Thin slices

Question 12

Stainings

Answer 12

Methylene blue
Acetin ocean
Sudan red
Iodine

Question 13

Methylene blue

Answer 13

All purpose

Question 14

Acetin orcein

Answer 14

Stains chromosomes red

Question 15

Sudan red

Answer 15

Lipids

Question 16

Iodine

Answer 16

Cellulose - yellow
Starch- blue black

Question 17

How to prepare a slide

Answer 17

Cut thin piece of tissue
Place on slide
Add a few drops of iodine to tissue
Place cover slip at an angle to prevent bubbles

Question 18

Rules of drawing

Answer 18

No shaded line
No sketchy lines
Must take up half the page
Label line No arrow head
Drawing lines must connect
Include scale bar

Question 19

Coarse adjustment knob

Answer 19

Move objective lense