Microscopy Flashcards
1
Q
Magnification
A
Number of times larger an image appears compared with the size of the object
2
Q
Resolution
A
The degree of detail visible
3
Q
Purpose of staininh
A
Makes cells visible
Makes organelles visible
Enables different type of cells to be distinguished
4
Q
Light microscope pros
A
Cheap
Easy to use
Observe living specimen
5
Q
TEM pros
A
High mag
High resolution
6
Q
SEM pros
A
High mag
High res
3d image
Surface
7
Q
TEM cons
A
Dead
Expensive
Need training to use
8
Q
TEM cons
A
Dead
Expensive
Need training to use
9
Q
How sem works
A
Electron beam is directed onto sample
10
Q
How tem works
A
Electron beam passes through sample
11
Q
How can specimen be preserved
A
Dehydrated
Embedded in wax
Thin slices
12
Q
Stainings
A
Methylene blue
Acetin ocean
Sudan red
Iodine
13
Q
Methylene blue
A
All purpose
14
Q
Acetin orcein
A
Stains chromosomes red
15
Q
Sudan red
A
Lipids
