DNA precipitation Flashcards
1
Q
What are all the things we need for this
A
Mortar pestle
Detergent
Protease enzyme
Salt
Ice cold ethanol
2
Q
Why do we use mortar and pestle
A
Break down cellulose cell wall
3
Q
Why do we add detergent
A
Dissolve cell surface membrane
4
Q
Why do we use protease enzyme
A
Break down the histone associated with DNA
5
Q
Why do we add salt
A
Break the hydrogen bonds between dna
6
Q
Why does the ethanol have to be ice cold
A
Reduce enzyme activity
7
Q
Why do we add ice cold ethanol
A
Causes precipitation breaks down cell surface membrane
