microscopy

Question 1

what is a eukaryotic cell

Answer 1

a cell with membrane bound cell organelles and in which the genetic material is enclosed in the nucleus.

Question 2

which lens determine the total magnification and resolution of a light microscope

Answer 2

the eyepiece and the objective lens

Question 3

difference between magnification and resolution

Answer 3

magnification is how much larger an image is than real life whereas resolution is the ability to distinguish between 2 adjacent indivisual points as seperate

Question 4

give an example of a eukaryotic cell

Answer 4

any plant, animal, fungal or protist cell.

Question 5

what kinds of specimen can be viewed under a light microscope?

Answer 5

dead or living whole cells, tissues or microorganisms.

Question 6

What are specimens tagged with, prior to viewing with a laser confocal microscope

Answer 6

Fluorescent tags

Question 7

Give an advantage of light microscopes

Answer 7

Cheap, easy to use, can view live specimen, lightweight

Question 8

why do specimens need to be stained?

Answer 8

to make them visible and to highlight distinguish between the cell structures

Question 9

what are the pros and cons of an electron microscope?

Answer 9

pros: very high magnification and excellent resolution
cons: specimen needs to be dead, it is very large, expensive and requires great skill and training to use.

Question 10

what are the maximum magnifications of microscopes

Answer 10

light microscope: x1500
TEM: x 1 000 000
SEM: x500 000

Question 11

what are the max resolutions of microscopes?

Answer 11

light microscope: 200 nm
TEM: 0.2 nm
SEM: 10nm